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Line 19: |
| **[[4q71]], [[4q72]], [[4q73]] – PutA (mutant) + FAD – ''Bradyrhizobium diazoefficiens''<br /> | | **[[4q71]], [[4q72]], [[4q73]] – PutA (mutant) + FAD – ''Bradyrhizobium diazoefficiens''<br /> |
| **[[4h6r]] – DrPutA + FAD – ''Deinococcus radiodurans''<br /> | | **[[4h6r]] – DrPutA + FAD – ''Deinococcus radiodurans''<br /> |
| | **[[2gpe]] - EcPutA DNA-binding domain - ''Escherichia coli''<br /> |
| | **[[2jxg]], [[2jxh]] - PpPutA DNA-binding domain - ''Pseudomonas putida'' - NMR<br /> |
| | **[[5ur2]] - PutA + FAD derivative - ''Bdellovibrio bacteriovorus''<br /> |
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| *PutA complex | | *PutA complex |
| Line 27: |
Line 30: |
| **[[4nme]] – GsPutA + FAD + propargylglycine <br /> | | **[[4nme]] – GsPutA + FAD + propargylglycine <br /> |
| **[[4nmf]] – GsPutA + FAD derivative + menadione bisulfite<br /> | | **[[4nmf]] – GsPutA + FAD derivative + menadione bisulfite<br /> |
| **[[1tiw]] – EcPutA PRODH domain + FAD + tetrahydrofuran derivative – ''Escherichia coli''<br /> | | **[[1tiw]] – EcPutA PRODH domain + FAD + tetrahydrofuran derivative <br /> |
| **[[3e2r]], [[4jny]], [[4jnz]] – EcPutA PRODH domain (mutant) + FAD + tetrahydrofuran derivative <br /> | | **[[3e2r]], [[4jny]], [[4jnz]] – EcPutA PRODH domain (mutant) + FAD + tetrahydrofuran derivative <br /> |
| **[[1tj0]], [[1tj1]] – EcPutA PRODH domain + FAD + lactate<br /> | | **[[1tj0]], [[1tj1]] – EcPutA PRODH domain + FAD + lactate<br /> |
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Line 41: |
| **[[4o8a]] – EcPutA DNA-binding + PRODH domains + FAD <br /> | | **[[4o8a]] – EcPutA DNA-binding + PRODH domains + FAD <br /> |
| **[[4h6q]] – DrPutA + FAD + THF <br /> | | **[[4h6q]] – DrPutA + FAD + THF <br /> |
| | **[[3haz]] - PutA + FAD + NAD - ''Bradyrhyzobium japonicum''<br /> |
| | **[[5ux5]] - PutA + FAD + NAD - ''Corynebacterium freiburgense''<br /> |
| | **[[5kf6]], [[5kf7]] - PutA + FAD + NAD + THF - ''Sinorhizobium meliloti''<br /> |
| | **[[2jxi]] - PpPutA DNA-binding domain + DNA<br /> |
| }} | | }} |
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| == References == | | == References == |
| <references/> | | <references/> |
| Function
Proline utilization A (PutA) is a bifunctional flavoprotein which acts as a transcriptional repressor of the put regulon or as a membrane-bound enzyme which catalyzes the oxidation of proline to glutamate. PutA domains include the DNA-binding domain (PRODH), the FAD-dependent proline dehydrogenase domain and the D-pyrroline-5-carboxylate dehydrogenase domain. The binding of proline to the PRODH active site and subsequent reduction of FAD causes a conformation change in PutA and enhance its membrane affinity. As a membrane-bound protein PutA switches from its repressor activity to its enzymatic role[1].
Structural highlights
The active site residue Tyr540 helps in substrate preference for proline over hydroxyproline. Water molecules shown as red spheres. The 3e2q structure displayed here contains the Tyr540Ser mutant[2].
- ↑ Srivastava D, Zhu W, Johnson WH, Whitman CP, Becker DF, Tanner JJ. The Structure of the Proline Utilization A Proline Dehydrogenase Domain Inactivated by N-Propargylglycine Provides Insight into Conformational Changes Induced by Substrate Binding and Flavin Reduction (,). Biochemistry. 2009 Dec 29. PMID:19994913 doi:10.1021/bi901717s
- ↑ Ostrander EL, Larson JD, Schuermann JP, Tanner JJ. A Conserved Active Site Tyrosine Residue of Proline Dehydrogenase Helps Enforce the Preference for Proline over Hydroxyproline as the Substrate (dagger) (double dagger). Biochemistry. 2009 Feb 10;48(5):951-9. PMID:19140736 doi:10.1021/bi802094k
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3D Structures of proline utilization A
Updated on 19-September-2017
{"openlevels":0}
- PutA
- 3e2q – GsPutA + FAD – Geobacter sulfurreducens
- 4nma – GsPutA + FAD + tetrahydrofuran derivative
- 2gpe – EcPutA DNA-binding domain
- 2ay0 – EcPutA DNA-binding domain (mutant)
- 4q71, 4q72, 4q73 – PutA (mutant) + FAD – Bradyrhizobium diazoefficiens
- 4h6r – DrPutA + FAD – Deinococcus radiodurans
- 2gpe - EcPutA DNA-binding domain - Escherichia coli
- 2jxg, 2jxh - PpPutA DNA-binding domain - Pseudomonas putida - NMR
- 5ur2 - PutA + FAD derivative - Bdellovibrio bacteriovorus
- PutA complex
- 4nmb – GsPutA + FAD + lactate
- 4nmc – GsPutA + FAD + zwittergent
- 4nmd – GsPutA + FAD + dithionite
- 4nme – GsPutA + FAD + propargylglycine
- 4nmf – GsPutA + FAD derivative + menadione bisulfite
- 1tiw – EcPutA PRODH domain + FAD + tetrahydrofuran derivative
- 3e2r, 4jny, 4jnz – EcPutA PRODH domain (mutant) + FAD + tetrahydrofuran derivative
- 1tj0, 1tj1 – EcPutA PRODH domain + FAD + lactate
- 1tj2 – EcPutA PRODH domain + FAD + acetate
- 2fzn – EcPutA PRODH domain + FAD + proline
- 3e2q, 3e2s – EcPutA PRODH domain (mutant) + FAD + proline derivative
- 2fzm – EcPutA PRODH domain + FAD + SO2
- 3itg – EcPutA PRODH domain + FAD + propargylglycine
- 2rbf – EcPutA DNA-binding domain + DNA
- 4o8a – EcPutA DNA-binding + PRODH domains + FAD
- 4h6q – DrPutA + FAD + THF
- 3haz - PutA + FAD + NAD - Bradyrhyzobium japonicum
- 5ux5 - PutA + FAD + NAD - Corynebacterium freiburgense
- 5kf6, 5kf7 - PutA + FAD + NAD + THF - Sinorhizobium meliloti
- 2jxi - PpPutA DNA-binding domain + DNA
References
