2j12: Difference between revisions

Revision as of 15:21, 12 October 2017

Ad37 fibre head in complex with CAR D1

Structural highlights

2j12 is a 2 chain structure with sequence from Adenovirus type 37 and Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	CA
Related:	1uxa, 1uxb, 1uxe, 1eaj, 1f5w, 1jew, 1kac, 1p69, 1p6a, 1rsf
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Adenovirus fibers from most serotypes bind the D1 domain of coxsackie and adenovirus receptor (CAR), although the binding residues are not strictly conserved. To understand this further, we determined the crystal structures of canine adenovirus serotype 2 (CAV-2) and the human adenovirus serotype 37 (HAd37) in complex with human CAR D1 at 2.3 and 1.5A resolution, respectively. Structure comparison with the HAd12 fiber head-CAR D1 complex showed that the overall topology of the interaction is conserved but that the interfaces differ in number and identity of interacting residues, shape complementarity, and degree of conformational adaptation. Using surface plasmon resonance, we characterized the binding affinity to CAR D1 of wild type and mutant CAV-2 and HAd37 fiber heads. We found that CAV-2 has the highest affinity but fewest direct interactions, with the reverse being true for HAd37. Moreover, we found that conserved interactions can have a minor contribution, whereas serotype-specific interactions can be essential. These results are discussed in the light of virus evolution and design of adenovirus vectors for gene transfer.

Structural and mutational analysis of human Ad37 and canine adenovirus 2 fiber heads in complex with the D1 domain of coxsackie and adenovirus receptor.,Seiradake E, Lortat-Jacob H, Billet O, Kremer EJ, Cusack S J Biol Chem. 2006 Nov 3;281(44):33704-16. Epub 2006 Aug 21. PMID:16923808^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Seiradake E, Lortat-Jacob H, Billet O, Kremer EJ, Cusack S. Structural and mutational analysis of human Ad37 and canine adenovirus 2 fiber heads in complex with the D1 domain of coxsackie and adenovirus receptor. J Biol Chem. 2006 Nov 3;281(44):33704-16. Epub 2006 Aug 21. PMID:16923808 doi:https://dx.doi.org/10.1074/jbc.M605316200

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OCA

[1] Seiradake E, Lortat-Jacob H, Billet O, Kremer EJ, Cusack S. Structural and mutational analysis of human Ad37 and canine adenovirus 2 fiber heads in complex with the D1 domain of coxsackie and adenovirus receptor. J Biol Chem. 2006 Nov 3;281(44):33704-16. Epub 2006 Aug 21. PMID:16923808 doi:https://dx.doi.org/10.1074/jbc.M605316200

[1]

@@ Line 1: / Line 1: @@
-==AD37 FIBRE HEAD IN COMPLEX WITH CAR D1==
+==Ad37 fibre head in complex with CAR D1==
 <StructureSection load='2j12' size='340' side='right' caption='[[2j12]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 == Structural highlights ==
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1uxa|1uxa]], [[1uxb|1uxb]], [[1uxe|1uxe]], [[1eaj|1eaj]], [[1f5w|1f5w]], [[1jew|1jew]], [[1kac|1kac]], [[1p69|1p69]], [[1p6a|1p6a]], [[1rsf|1rsf]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j12 OCA], [http://pdbe.org/2j12 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j12 RCSB], [http://www.ebi.ac.uk/pdbsum/2j12 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j12 OCA], [http://pdbe.org/2j12 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j12 RCSB], [http://www.ebi.ac.uk/pdbsum/2j12 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j12 ProSAT]</span></td></tr>
 </table>
-== Function ==
-[[http://www.uniprot.org/uniprot/CXAR_HUMAN CXAR_HUMAN]] Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN.<ref>PMID:9096397</ref> <ref>PMID:11734628</ref> <ref>PMID:12297051</ref> <ref>PMID:15800062</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 35: / Line 34: @@
 [[Category: Human]]
 [[Category: Billet, O]]
-[[Category: Cusac, S]]
+[[Category: Cusack, S]]
 [[Category: Kremer, E J]]
 [[Category: Lortat-Jacob, H]]
@@ Line 57: / Line 56: @@
 [[Category: Transmembrane]]
 [[Category: Viral protein-receptor complex]]
-[[Category: Viral protein/receptor]]

2j12: Difference between revisions

Revision as of 15:21, 12 October 2017

Ad37 fibre head in complex with CAR D1

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

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