5nol

Ca2+-induced Movement of Tropomyosin on Native Cardiac Thin Filaments - "Closed" state

spinqualitylabelsall models 5nol, resolution 8.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5nol is a 7 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ADP, MG NonStd Res: UNK Related: 5nog, 5noj Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Muscle contraction relies on the interaction of myosin motors with F-actin, which is regulated through a translocation of tropomyosin by the troponin complex in response to Ca2+ The current model of muscle regulation holds that at relaxing (low-Ca2+) conditions tropomyosin blocks myosin binding sites on F-actin, whereas at activating (high-Ca2+) conditions tropomyosin translocation only partially exposes myosin binding sites on F-actin so that binding of rigor myosin is required to fully activate the thin filament (TF). Here we used a single-particle approach to helical reconstruction of frozen hydrated native cardiac TFs under relaxing and activating conditions to reveal the azimuthal movement of the tropomyosin on the surface of the native cardiac TF upon Ca2+ activation. We demonstrate that at either relaxing or activating conditions tropomyosin is not constrained in one structural state, but rather is distributed between three structural positions on the surface of the TF. We show that two of these tropomyosin positions restrain actomyosin interactions, whereas in the third position, which is significantly enhanced at high Ca2+, tropomyosin does not block myosin binding sites on F-actin. Our data provide a structural framework for the enhanced activation of the cardiac TF over the skeletal TF by Ca2+ and lead to a mechanistic model for the regulation of the cardiac TF. Ca2+-induced movement of tropomyosin on native cardiac thin filaments revealed by cryoelectron microscopy.,Risi C, Eisner J, Belknap B, Heeley DH, White HD, Schroder GF, Galkin VE Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):6782-6787. doi:, 10.1073/pnas.1700868114. Epub 2017 Jun 12. PMID:28607071 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Tropomyosin

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