5wda: Difference between revisions
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==Structure of the PulG pseudopilus== | |||
<StructureSection load='5wda' size='340' side='right' caption='[[5wda]], [[Resolution|resolution]] 5.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5wda]] is a 25 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WDA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WDA FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MEA:N-METHYLPHENYLALANINE'>MEA</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5o2y|5o2y]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wda FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wda OCA], [http://pdbe.org/5wda PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wda RCSB], [http://www.ebi.ac.uk/pdbsum/5wda PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wda ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Many Gram-negative bacteria use type 2 secretion systems (T2SSs) to secrete proteins involved in virulence and adaptation. Transport of folded proteins via T2SS nanomachines requires the assembly of inner membrane-anchored fibres called pseudopili. Although efficient pseudopilus assembly is essential for protein secretion, structure-based functional analyses are required to unravel the mechanistic link between these processes. Here, we report an atomic model for a T2SS pseudopilus from Klebsiella oxytoca, obtained by fitting the NMR structure of its calcium-bound subunit PulG into the ~5-A-resolution cryo-electron microscopy reconstruction of assembled fibres. This structure reveals the comprehensive network of inter-subunit contacts and unexpected features, including a disordered central region of the PulG helical stem, and highly flexible C-terminal residues on the fibre surface. NMR, mutagenesis and functional analyses highlight the key role of calcium in PulG folding and stability. Fibre disassembly in the absence of calcium provides a basis for pseudopilus length control, essential for protein secretion, and supports the Archimedes screw model for the type 2 secretion mechanism.An atomic model for a type 2 secretion system pseudopilus from Klebsiella oxytoca reveals a comprehensive network of inter-subunit contacts while mutational and functional analyses highlight the role of calcium in PulG folding and stability. | |||
Structure of the calcium-dependent type 2 secretion pseudopilus.,Lopez-Castilla A, Thomassin JL, Bardiaux B, Zheng W, Nivaskumar M, Yu X, Nilges M, Egelman EH, Izadi-Pruneyre N, Francetic O Nat Microbiol. 2017 Oct 9. doi: 10.1038/s41564-017-0041-2. PMID:28993624<ref>PMID:28993624</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5wda" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bardiaux, B]] | |||
[[Category: Egelman, E H]] | |||
[[Category: Francetic, O]] | |||
[[Category: Izadi-Pruneyre, N]] | |||
[[Category: Lopez-Castilla, A]] | |||
[[Category: Nilges, M]] | |||
[[Category: Nivaskumar, M]] | |||
[[Category: Thomassin, J L]] | |||
[[Category: Yu, X]] | |||
[[Category: Zheng, W]] | |||
[[Category: Bacterial secretion]] | |||
[[Category: Cryo-em]] | |||
[[Category: Helical polymer]] | |||
[[Category: Protein transport]] | |||
Revision as of 06:59, 25 October 2017
Structure of the PulG pseudopilus
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