5wos: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 5wos is ~~ON HOLD~~ ~~until Paper~~ Publication

==Structural and functional insights into Canarypox Virus CNP058 regulation of apoptosis==

== Structural highlights ==

<table><tr><td colspan='2'>[[5wos]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WOS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WOS FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wos OCA], [http://pdbe.org/5wos PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wos RCSB], [http://www.ebi.ac.uk/pdbsum/5wos PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wos ProSAT]</span></td></tr>

</table>

== Function ==

[[http://www.uniprot.org/uniprot/B2L11_HUMAN B2L11_HUMAN]] Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.<ref>PMID:9430630</ref> <ref>PMID:11734221</ref> <ref>PMID:12019181</ref> <ref>PMID:11997495</ref> <ref>PMID:15147734</ref> <ref>PMID:15486195</ref>

== Publication Abstract from PubMed ==

Programmed cell death or apoptosis is an important component of host defense systems against viral infection. The B-cell lymphoma 2 (Bcl-2) proteins family is the main arbiter of mitochondrially mediated apoptosis, and viruses have evolved sequence and structural mimics of Bcl-2 to subvert premature host cell apoptosis in response to viral infection. The sequencing of the canarypox virus genome identified a putative pro-survival Bcl-2 protein, CNP058. However, a role in apoptosis inhibition for CNP058 has not been identified to date. Here, we report that CNP058 is able to bind several host cell pro-death Bcl-2 proteins, including Bak and Bax, as well as several BH3 only-proteins including Bim, Bid, Bmf, Noxa, Puma, and Hrk with high to moderate affinities. We then defined the structural basis for CNP058 binding to pro-death Bcl-2 proteins by determining the crystal structure of CNP058 bound to Bim BH3. CNP058 adopts the conserved Bcl-2 like fold observed in cellular pro-survival Bcl-2 proteins, and utilizes the canonical ligand binding groove to bind Bim BH3. We then demonstrate that CNP058 is a potent inhibitor of ultraviolet (UV) induced apoptosis in a cell culture model. Our findings suggest that CNP058 is a potent inhibitor of apoptosis that is able to bind to BH3 domain peptides from a broad range of pro-death Bcl-2 proteins, and may play a key role in countering premature host apoptosis.

~~Authors:~~ Anasir, M.I.~~, Kvansakul, M~~.

Structural and Functional Insight into Canarypox Virus CNP058 Mediated Regulation of Apoptosis.,Anasir MI, Baxter AA, Poon IKH, Hulett MD, Kvansakul M Viruses. 2017 Oct 20;9(10). pii: E305. doi: 10.3390/v9100305. PMID:29053589<ref>PMID:29053589</ref>

~~Description: Structural and functional insights into Canarypox Virus CNP058 regulation~~ of ~~apoptosis~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

~~[[Category~~: ~~Unreleased Structures]]~~

</div>

[[Category: Anasir, M.I]]

== References ==

__TOC__

</StructureSection>

[[Category: Anasir, M I]]

[[Category: Kvansakul, M]]

[[Category: Apoptosis]]

[[Category: Avipoxvirus]]

[[Category: Bcl-2]]

[[Category: Bim]]

[[Category: Viral protein]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5wos is ON HOLD  until Paper Publication
+==Structural and functional insights into Canarypox Virus CNP058 regulation of apoptosis==
+<StructureSection load='5wos' size='340' side='right' caption='[[5wos]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5wos]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WOS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WOS FirstGlance]. <br>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wos OCA], [http://pdbe.org/5wos PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wos RCSB], [http://www.ebi.ac.uk/pdbsum/5wos PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wos ProSAT]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/B2L11_HUMAN B2L11_HUMAN]] Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.<ref>PMID:9430630</ref> <ref>PMID:11734221</ref> <ref>PMID:12019181</ref> <ref>PMID:11997495</ref> <ref>PMID:15147734</ref> <ref>PMID:15486195</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Programmed cell death or apoptosis is an important component of host defense systems against viral infection. The B-cell lymphoma 2 (Bcl-2) proteins family is the main arbiter of mitochondrially mediated apoptosis, and viruses have evolved sequence and structural mimics of Bcl-2 to subvert premature host cell apoptosis in response to viral infection. The sequencing of the canarypox virus genome identified a putative pro-survival Bcl-2 protein, CNP058. However, a role in apoptosis inhibition for CNP058 has not been identified to date. Here, we report that CNP058 is able to bind several host cell pro-death Bcl-2 proteins, including Bak and Bax, as well as several BH3 only-proteins including Bim, Bid, Bmf, Noxa, Puma, and Hrk with high to moderate affinities. We then defined the structural basis for CNP058 binding to pro-death Bcl-2 proteins by determining the crystal structure of CNP058 bound to Bim BH3. CNP058 adopts the conserved Bcl-2 like fold observed in cellular pro-survival Bcl-2 proteins, and utilizes the canonical ligand binding groove to bind Bim BH3. We then demonstrate that CNP058 is a potent inhibitor of ultraviolet (UV) induced apoptosis in a cell culture model. Our findings suggest that CNP058 is a potent inhibitor of apoptosis that is able to bind to BH3 domain peptides from a broad range of pro-death Bcl-2 proteins, and may play a key role in countering premature host apoptosis.
-Authors: Anasir, M.I., Kvansakul, M.
+Structural and Functional Insight into Canarypox Virus CNP058 Mediated Regulation of Apoptosis.,Anasir MI, Baxter AA, Poon IKH, Hulett MD, Kvansakul M Viruses. 2017 Oct 20;9(10). pii: E305. doi: 10.3390/v9100305. PMID:29053589<ref>PMID:29053589</ref>
-Description: Structural and functional insights into Canarypox Virus CNP058 regulation of apoptosis
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Anasir, M.I]]
+<div class="pdbe-citations 5wos" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Anasir, M I]]
 [[Category: Kvansakul, M]]
+[[Category: Apoptosis]]
+[[Category: Avipoxvirus]]
+[[Category: Bcl-2]]
+[[Category: Bim]]
+[[Category: Viral protein]]