5npg: Difference between revisions
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<StructureSection load='5npg' size='340' side='right' caption='[[5npg]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | <StructureSection load='5npg' size='340' side='right' caption='[[5npg]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5npg]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NPG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NPG FirstGlance]. <br> | <table><tr><td colspan='2'>[[5npg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NPG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NPG FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5npg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5npg OCA], [http://pdbe.org/5npg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5npg RCSB], [http://www.ebi.ac.uk/pdbsum/5npg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5npg ProSAT]</span></td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">loqs, Dmel\CG6866, dRax, loq, LOQS, Loqs, R3D1, r3d1, R3D1-L, R3D1-S, TRBP, CG6866, Dmel_CG6866 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5npg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5npg OCA], [http://pdbe.org/5npg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5npg RCSB], [http://www.ebi.ac.uk/pdbsum/5npg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5npg ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
RNA interference defends against RNA viruses and retro-elements within an organism's genome. It is triggered by duplex siRNAs, of which one strand is selected to confer sequence-specificity to the RNA induced silencing complex (RISC). In Drosophila, Dicer-2 (Dcr-2) and the double-stranded RNA binding domain (dsRBD) protein R2D2 form the RISC loading complex (RLC) and select one strand of exogenous siRNAs according to the relative thermodynamic stability of base-pairing at either end. Through genome editing we demonstrate that Loqs-PD, the Drosophila homolog of human TAR RNA binding protein (TRBP) and a paralog of R2D2, forms an alternative RLC with Dcr-2 that is required for strand choice of endogenous siRNAs in S2 cells. Two canonical dsRBDs in Loqs-PD bind to siRNAs with enhanced affinity compared to miRNA/miRNA* duplexes. Structural analysis, NMR and biophysical experiments indicate that the Loqs-PD dsRBDs can slide along the RNA duplex to the ends of the siRNA. A moderate but notable binding preference for the thermodynamically more stable siRNA end by Loqs-PD alone is greatly amplified in complex with Dcr-2 to initiate strand discrimination by asymmetry sensing in the RLC. | |||
Molecular basis for asymmetry sensing of siRNAs by the Drosophila Loqs-PD/Dcr-2 complex in RNA interference.,Tants JN, Fesser S, Kern T, Stehle R, Geerlof A, Wunderlich C, Juen M, Hartlmuller C, Bottcher R, Kunzelmann S, Lange O, Kreutz C, Forstemann K, Sattler M Nucleic Acids Res. 2017 Oct 13. doi: 10.1093/nar/gkx886. PMID:29040648<ref>PMID:29040648</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5npg" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Drome]] | |||
[[Category: Boettcher, R]] | [[Category: Boettcher, R]] | ||
[[Category: Fesser, S]] | [[Category: Fesser, S]] | ||
Revision as of 06:17, 1 November 2017
Solution structure of Drosophila melanogaster Loquacious dsRBD1
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