5jxd: Difference between revisions
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==Crystal structure of murine Tnfaip8 C165S mutant== | |||
<StructureSection load='5jxd' size='340' side='right' caption='[[5jxd]], [[Resolution|resolution]] 2.03Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5jxd]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JXD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JXD FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6OU:[(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl]+(~{Z})-octadec-9-enoate'>6OU</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jxd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jxd OCA], [http://pdbe.org/5jxd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jxd RCSB], [http://www.ebi.ac.uk/pdbsum/5jxd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jxd ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/TFIP8_MOUSE TFIP8_MOUSE]] Acts as a negative mediator of apoptosis. Suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Defective hepatic autophagy is observed in obesity and diabetes, whereas autophagy is inhibited by insulin in hepatocytes. Insulin-induced anti-autophagy is mediated by non-canonical Galphai3 signaling via an unknown mechanism. Previously, we identified the anti-autophagic activity of Tnfaip8 via activation of mammalian target of rapamycin (mTOR) in the nervous system. Here, we demonstrate that insulin temporally induces Tnfaip8, which mediates the anti-autophagic action of insulin through formation of a novel ternary complex including Tnfaip8, phosphatidylethanolamine (PE) and Galphai3. Specifically, an X-ray crystallographic study of Tnfaip8 from Mus musculus (mTnfaip8) at 2.03 A together with LC-MS analyses reveals PE in the hydrophobic cavity. However, an mTnfaip8 mutant lacking PE does not interact with Galphai3, indicating that the PE component is critical for the anti-autophagic action of mTnfaip8 via interaction with Galphai3. Therefore, the mTnfaip8-PE complex may act as an essential upstream effector via ternary complex formation most likely with active Galphai3 during insulin-induced anti-autophagy. | |||
The Tnfaip8-PE complex is a novel upstream effector in the anti-autophagic action of insulin.,Kim JS, Park J, Kim MS, Ha JY, Jang YW, Shin DH, Son JH Sci Rep. 2017 Jul 24;7(1):6248. doi: 10.1038/s41598-017-06576-3. PMID:28740220<ref>PMID:28740220</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: Kim, M | <div class="pdbe-citations 5jxd" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Kim, M S]] | |||
[[Category: Lee, D]] | [[Category: Lee, D]] | ||
[[Category: Park, J]] | [[Category: Park, J]] | ||
[[Category: Shin, D H]] | |||
[[Category: Immune system]] | |||
[[Category: Oxi-a]] | |||
[[Category: Phosphatidylethanolamine]] | |||
[[Category: Tnfaip8]] | |||
Revision as of 06:18, 1 November 2017
Crystal structure of murine Tnfaip8 C165S mutant
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