3nok

Crystal structure of Myxococcus xanthus Glutaminyl Cyclase

spinqualitylabelsall models 3nok, resolution 1.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3nok is a 2 chain structure with sequence from Atcc 25232. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA, DDQ, GOL, MES Activity: Glutaminyl-peptide cyclotransferase, with EC number 2.3.2.5 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Abstract Although enzymes responsible for the cyclization of amino terminal glutamine residues are present in both plant and mammal species, none have yet been characterized in bacteria. Based on low sequence homologies to plant glutaminyl cyclases (QC), we cloned the coding sequences of putative microbial QCs from Zymomonas mobilis (ZmQC) and Myxococcus xanthus (MxQC). The two recombinant enzymes exhibited distinct QC activity, with specificity constants k(cat)/K(m) of 1.47+/-0.33 mm(-1)s(-1) (ZmQC) and 142+/-32.7 mm(-1)s(-1) (MxQC) towards the fluorescent substrate glutamine-7-amino-4-methyl-coumarine. The measured pH-rate profile of the second order rate constant displayed an interesting deviation towards the acidic limb of the pH chart in the case of ZmQC, while MxQC showed maximum activity in the mild alkaline pH range. Analysis of the enzyme variants ZmQCGlu(46)Gln and MxQCGln(46)Glu show that the exchanged residues play a significant role in the pH behaviour of the respective enzymes. In addition, we determined the three dimensional crystal structures of both enzymes. The tertiary structure is defined by a five-bladed beta-propeller anchored by a core cation. The structures corroborate the putative location of the active site and confirm the proposed relationship between bacterial and plant glutaminyl cyclases. Kinetic and structural characterization of bacterial glutaminyl cyclases from Zymomonas mobilis and Myxococcus xanthus.,Carrillo DR, Parthier C, Janckel N, Grandke J, Stelter M, Schilling S, Boehme M, Neumann P, Wolf R, Demuth HU, Stubbs MT, Rahfeld JU Biol Chem. 2010 Sep 24. PMID:20868223 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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