3pkg

Urate oxidase under 2 MPa / 20 bars pressure of xenon

spinqualitylabelsall models 3pkg, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3pkg is a 1 chain structure with sequence from Aspfl. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: AZA, NA, XE NonStd Res: ACE Related: 3pjk, 3pk3, 3pk4, 3pk5, 3pk6, 3pk8, 3pkf, 3pkh, 3pkk, 3pkl, 3pks, 3pkt, 3pku, 3ple, 3plg, 3plh, 3pli, 3plj, 3plm, 2ic0, 2icq, 2iba Gene: uaZ, uox (ASPFL) Activity: Factor independent urate hydroxylase, with EC number 1.7.3.3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [URIC_ASPFL] Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin. Publication Abstract from PubMed The remarkably safe anesthetics xenon (Xe) and, to lesser extent, nitrous oxide (N(2)O) possess neuroprotective properties in preclinical studies. To investigate the mechanisms of pharmacological action of these gases, which are still poorly known, we performed both crystallography under a large range of gas pressure and biochemical studies on urate oxidase, a prototype of globular gas-binding proteins whose activity is modulated by inert gases. We show that Xe and N(2)O bind to, compete for, and expand the volume of a hydrophobic cavity located just behind the active site of urate oxidase and further inhibit urate oxidase enzymatic activity. By demonstrating a significant relationship between the binding and biochemical effects of Xe and N(2)O, given alone or in combination, these data from structure to function highlight the mechanisms by which chemically and metabolically inert gases can alter protein function and produce their pharmacological effects. Interestingly, the effects of a Xe:N(2)O equimolar mixture were found to be equivalent to those of Xe alone, thereby suggesting that gas mixtures containing Xe and N(2)O could be an alternative and efficient neuroprotective strategy to Xe alone, whose widespread clinical use is limited due to the cost of production and availability of this gas.-Marassio, G., Prange, T., David, H. N., Sopkova-de Oliveira Santos, J., Gabison, L., Delcroix, N., Abraini, J. H., Colloc'h, N. Pressure-response analysis of anesthetic gases xenon and nitrous oxide on urate oxidase: a crystallographic study. Pressure-response analysis of anesthetic gases xenon and nitrous oxide on urate oxidase: a crystallographic study.,Marassio G, Prange T, David HN, Sopkova-de Oliveira Santos J, Gabison L, Delcroix N, Abraini JH, Colloc'h N FASEB J. 2011 Mar 18. PMID:21421845 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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