4duq

The Structure of Ca2+-loaded S100A2 at 1.3A resolution

spinqualitylabelsall models 4duq, resolution 1.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4duq is a 2 chain structure with sequence from Human. This structure supersedes the now removed PDB entry 3n22. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 15P, CA Gene: S100A2, S100L (HUMAN) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [S10A2_HUMAN] May act as a modulator against excess calcium accumulation in normal human mammary epithelial cells. May also play a role in suppressing tumor cell growth. Publication Abstract from PubMed S100A2 is an EF-hand Ca(2+) -binding protein that activates the tumor suppressor p53. In order to understand the molecular mechanisms underlying the Ca(2+) -induced activation of S100A2 the structure of Ca(2+) -bound S100A2 was determined at 1.3 A resolution by X-ray crystallography. The structure was compared to Ca(2+) -free S100A2 and to other S100 proteins. Ca(2+) binding to S100A2 induces small structural changes in the N-terminal EF-hand, but a large conformational change in the C-terminal EF-hand reorienting helix III by approximately 90 degrees is observed. This movement is accompanied by the exposure of a hydrophobic cavity between helix III and helix IV that represents the target protein interaction site. This molecular reorganization is associated with the breaking and new formation of intramolecular hydrophobic contacts. The target binding site exhibits unique features; in particular the hydrophobic cavity is larger than in other Ca(2+) -loaded S100 proteins. The structural data underline that the shape and size of the hydrophobic cavity are major determinants for target specificity of S100 proteins and suggests that the binding mode for S100A2 is different from other p53-interacting S100 proteins. STRUCTURED DIGITAL ABSTRACT: S100A2 and S100A2bind by x-ray crystallography (View interaction). The Structure of Ca(2+) -loaded S100A2 at 1.3 A resolution.,Koch M, Fritz G FEBS J. 2012 Mar 6. doi: 10.1111/j.1742-4658.2012.08556.x. PMID:22394450 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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