Structural highlights
3t72 is a 50 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Gene: | phoB, b0399, JW0389 (ECOLI), rpoD, alt, b3067, JW3039, EKO11_4334, rpoB (ECOLI) |
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[RPOD_ECOLI] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium. [PHOB_ECOLI] This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.
Publication Abstract from PubMed
PhoB is a two-component response regulator that activates transcription by interacting with the sigma(70) subunit of the E. coli RNA polymerase in promoters in which the -35 sigma(70)-recognition element is replaced by the pho box. The crystal structure of a transcription initiation subcomplex that includes the sigma(4) domain of sigma(70) fused with the RNA polymerase beta subunit flap tip helix, the PhoB effector domain and the pho box DNA reveals how sigma(4) recognizes the upstream pho box repeat. As with the -35 element, sigma(4) achieves this recognition through the N-terminal portion of its DNA recognition helix, but contact with the DNA major groove is less extensive. Unexpectedly, the same recognition helix contacts the transactivation loop and helices alpha2 and alpha3 of PhoB. This result shows a simple and elegant mechanism for polymerase recruitment to pho box promoters in which the lost -35 element contacts are compensated by new ones with the activator. In addition, sigma(4) is reoriented, thereby suggesting a remodelling mechanism for transcription initiation.
The structure of a transcription activation subcomplex reveals how sigma(70) is recruited to PhoB promoters.,Blanco AG, Canals A, Bernues J, Sola M, Coll M EMBO J. 2011 Aug 9;30(18):3776-85. doi: 10.1038/emboj.2011.271. PMID:21829166[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Blanco AG, Canals A, Bernues J, Sola M, Coll M. The structure of a transcription activation subcomplex reveals how sigma(70) is recruited to PhoB promoters. EMBO J. 2011 Aug 9;30(18):3776-85. doi: 10.1038/emboj.2011.271. PMID:21829166 doi:10.1038/emboj.2011.271
