5m2o: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 5m2o is ~~ON HOLD until Paper~~ Publication

==R. flavefaciens' third ScaB cohesin in complex with a group 1 dockerin==

== Structural highlights ==

<table><tr><td colspan='2'>[[5m2o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ruminococcus_flavefaciens_fd-1 Ruminococcus flavefaciens fd-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M2O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M2O FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5aoz|5aoz]]</td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">scaB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=641112 Ruminococcus flavefaciens FD-1])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m2o OCA], [http://pdbe.org/5m2o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m2o RCSB], [http://www.ebi.ac.uk/pdbsum/5m2o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m2o ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

ABTRACT: Cellulosomes are sophisticated multi-enzymatic nanomachines produced by anaerobes to effectively deconstruct plant structural carbohydrates. Cellulosome assembly involves the binding of enzyme-borne dockerins (Doc) to repeated cohesin (Coh) modules located in a non-catalytic scaffoldin. Docs appended to cellulosomal enzymes generally present two similar Coh-binding interfaces supporting a dual-binding mode, which may confer increased positional adjustment of the different complex components. Ruminococcus flavefaciens' cellulosome is assembled from a repertoire of 223 Doc-containing proteins classified into 6 groups. Recent studies revealed that Docs of groups 3 and 6 are recruited to the cellulosome via a single-binding mode mechanism with an adaptor scaffoldin. To investigate the extent to which the single-binding mode contributes to the assembly of R. flavefaciens cellulosome, the structures of two group 1 Docs bound to Cohs of primary (ScaA) and adaptor (ScaB) scaffoldins were solved. The data revealed that group 1 Docs display a conserved mechanism of Coh recognition involving a single-binding mode. Therefore, in contrast to all cellulosomes described to date, the assembly of R. flavefaciens cellulosome involves single but not dual-binding mode Docs. Thus, this work reveals a novel mechanism of cellulosome assembly and challenges the ubiquitous implication of the dual-binding mode in the acquisition of cellulosome flexibility.

~~Authors:~~ Bule, P., ~~Najmudin~~, S., Carvalho, ~~A.L.~~, Fontes~~, C~~.M.G.A.

Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes.,Bule P, Alves VD, Israeli-Ruimy V, Carvalho AL, Ferreira LM, Smith SP, Gilbert HJ, Najmudin S, Bayer EA, Fontes CM Sci Rep. 2017 Apr 7;7(1):759. doi: 10.1038/s41598-017-00919-w. PMID:28389644<ref>PMID:28389644</ref>

~~Description~~: R. flavefaciens~~' third ScaB cohesin in complex with a group~~ 1 ~~dockerin~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased Structures~~]]

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Ruminococcus flavefaciens fd-1]]

[[Category: Bule, P]]

[[Category: Carvalho, A L]]

[[Category: Fontes, C M.G A]]

[[Category: Najmudin, S]]

[[Category: ~~Bule, P~~]]

[[Category: Cellulosome]]

[[Category: ~~Carvalho, A~~.L]]

[[Category: Cohesin]]

[[Category: ~~Fontes, C.M.G.A~~]]

[[Category: Complex]]

[[Category: Dockerin]]

[[Category: Protein binding]]

[[Category: R. flavefacien]]

[[Category: Structural protein]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5m2o is ON HOLD  until Paper Publication
+==R. flavefaciens' third ScaB cohesin in complex with a group 1 dockerin==
+<StructureSection load='5m2o' size='340' side='right' caption='[[5m2o]], [[Resolution|resolution]] 1.26&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5m2o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ruminococcus_flavefaciens_fd-1 Ruminococcus flavefaciens fd-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M2O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M2O FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5aoz|5aoz]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">scaB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=641112 Ruminococcus flavefaciens FD-1])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m2o OCA], [http://pdbe.org/5m2o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m2o RCSB], [http://www.ebi.ac.uk/pdbsum/5m2o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m2o ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+ABTRACT: Cellulosomes are sophisticated multi-enzymatic nanomachines produced by anaerobes to effectively deconstruct plant structural carbohydrates. Cellulosome assembly involves the binding of enzyme-borne dockerins (Doc) to repeated cohesin (Coh) modules located in a non-catalytic scaffoldin. Docs appended to cellulosomal enzymes generally present two similar Coh-binding interfaces supporting a dual-binding mode, which may confer increased positional adjustment of the different complex components. Ruminococcus flavefaciens' cellulosome is assembled from a repertoire of 223 Doc-containing proteins classified into 6 groups. Recent studies revealed that Docs of groups 3 and 6 are recruited to the cellulosome via a single-binding mode mechanism with an adaptor scaffoldin. To investigate the extent to which the single-binding mode contributes to the assembly of R. flavefaciens cellulosome, the structures of two group 1 Docs bound to Cohs of primary (ScaA) and adaptor (ScaB) scaffoldins were solved. The data revealed that group 1 Docs display a conserved mechanism of Coh recognition involving a single-binding mode. Therefore, in contrast to all cellulosomes described to date, the assembly of R. flavefaciens cellulosome involves single but not dual-binding mode Docs. Thus, this work reveals a novel mechanism of cellulosome assembly and challenges the ubiquitous implication of the dual-binding mode in the acquisition of cellulosome flexibility.
-Authors: Bule, P., Najmudin, S., Carvalho, A.L., Fontes, C.M.G.A.
+Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes.,Bule P, Alves VD, Israeli-Ruimy V, Carvalho AL, Ferreira LM, Smith SP, Gilbert HJ, Najmudin S, Bayer EA, Fontes CM Sci Rep. 2017 Apr 7;7(1):759. doi: 10.1038/s41598-017-00919-w. PMID:28389644<ref>PMID:28389644</ref>
-Description: R. flavefaciens' third ScaB cohesin in complex with a group 1 dockerin
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 5m2o" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Ruminococcus flavefaciens fd-1]]
+[[Category: Bule, P]]
+[[Category: Carvalho, A L]]
+[[Category: Fontes, C M.G A]]
 [[Category: Najmudin, S]]
-[[Category: Bule, P]]
+[[Category: Cellulosome]]
-[[Category: Carvalho, A.L]]
+[[Category: Cohesin]]
-[[Category: Fontes, C.M.G.A]]
+[[Category: Complex]]
+[[Category: Dockerin]]
+[[Category: Protein binding]]
+[[Category: R. flavefacien]]
+[[Category: Structural protein]]