4rbq: Difference between revisions
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==32 base pair oligo(U) RNA== | |||
<StructureSection load='4rbq' size='340' side='right' caption='[[4rbq]], [[Resolution|resolution]] 1.05Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4rbq]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RBQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RBQ FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3nd3|3nd3]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rbq OCA], [http://pdbe.org/4rbq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rbq RCSB], [http://www.ebi.ac.uk/pdbsum/4rbq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rbq ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Head-to-head fusions of two identical double-stranded fragments of RNA can be designed to self-assemble from a single RNA species and form a double-stranded helix with a twofold rotation axis relating the two strands. These symmetrical RNA molecules are more likely to crystallize without end-on-end statistical packing disorder because the two halves of the molecule are identical. This approach can be used to study many fragments of double-stranded RNA or many isolated helical domains from large single-stranded RNAs that may not yet be amenable to high-resolution studies by crystallography or NMR. We used fusion RNAs to study one (the U-helix) of three functional domains formed when guide RNA binds to its cognate pre-edited mRNA from the trypanosome RNA editing system. The U-helix forms when the 3' oligo(U) tail of the guide RNA (gRNA) binds to the purine-rich, pre-edited mRNA upstream from the current RNA editing site. Fusion RNAs 16-and 32-base pairs in length formed crystals that gave diffraction to 1.37 and 1.05 A respectively. We provide the composition of a fusion RNA crystallization screen and describe the X-ray data collection, structure determination, and refinement of the crystal structures of fusion RNAs. | |||
Fusion RNAs in crystallographic studies of double-stranded RNA from trypanosome RNA editing.,Mooers BH Methods Mol Biol. 2015;1240:191-216. doi: 10.1007/978-1-4939-1896-6_14. PMID:25352146<ref>PMID:25352146</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4rbq" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Mooers, B H.M]] | |||
[[Category: 3' u-tail]] | |||
[[Category: A-form rna]] | |||
[[Category: Atomic resolution]] | |||
[[Category: Double helix]] | |||
[[Category: Mitochondrion]] | |||
[[Category: Oligou]] | |||
[[Category: Rna]] | |||
[[Category: Trypanosome rna editing substrate]] | |||
