4xwu

Structure of the IMP dehydrogenase from Ashbya gossypii

spinqualitylabelsall models 4xwu, resolution 1.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4xwu is a 1 chain structure with sequence from Ashgo. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: AGOS_AER117W (ASHGO) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [Q756Z6_ASHGO] Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.[HAMAP-Rule:MF_03156] Publication Abstract from PubMed Guanine nucleotides are the precursors of essential biomolecules including nucleic acids and vitamins such as riboflavin. The enzyme inosine-5'-monophosphate dehydrogenase (IMPDH) catalyzes the ratelimiting step in the guanine nucleotide de novo biosynthetic pathway and plays a key role in controlling the cellular nucleotide pools. Thus, IMPDH is an important metabolic bottleneck in the guanine nucleotide synthesis, susceptible of manipulation by means of metabolic engineering approaches. Herein, we report the functional and structural characterization of the IMPDH enzyme from the industrial fungus Ashbya gossypii. Our data show that the overexpression of the IMPDH gene increases the metabolic flux through the guanine pathway and ultimately enhances 40 % riboflavin production with respect to the wild type. Also, IMPDH disruption results in a 100-fold increase of inosine excretion to the culture media. Our results contribute to the developing metabolic engineering toolbox aiming at improving the production of metabolites with biotechnological interest in A. gossypii. Increased riboflavin production by manipulation of inosine 5'-monophosphate dehydrogenase in Ashbya gossypii.,Buey RM, Ledesma-Amaro R, Balsera M, de Pereda JM, Revuelta JL Appl Microbiol Biotechnol. 2015 Jul 7. PMID:26150243 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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