5cr8: Difference between revisions

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'''Unreleased structure'''


The entry 5cr8 is ON HOLD  until Paper Publication
==Structure of the membrane-binding domain of pneumolysin==
<StructureSection load='5cr8' size='340' side='right' caption='[[5cr8]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5cr8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CR8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CR8 FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ply ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 "Diplococcus pneumoniae" (Klein 1884) Weichselbaum 1886])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cr8 OCA], [http://pdbe.org/5cr8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cr8 RCSB], [http://www.ebi.ac.uk/pdbsum/5cr8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cr8 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/A4GRG6_STREE A4GRG6_STREE]] Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex.[RuleBase:RU364025]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Pneumolysin is a cholesterol-dependent cytolysin (CDC) and virulence factor of Streptococcus pneumoniae. It kills cells by forming pores assembled from oligomeric rings in cholesterol-containing membranes. Cryo-EM has revealed the structures of the membrane-surface bound pre-pore and inserted-pore oligomers, however the molecular contacts that mediate these oligomers are unknown because high-resolution information is not available. Here we have determined the crystal structure of full-length pneumolysin at 1.98 A resolution. In the structure, crystal contacts demonstrate the likely interactions that enable polymerisation on the cell membrane and the molecular packing of the pre-pore complex. The hemolytic activity is abrogated in mutants that disrupt these intermolecular contacts, highlighting their importance during pore formation. An additional crystal structure of the membrane-binding domain alone suggests that changes in the conformation of a tryptophan rich-loop at the base of the toxin promote monomer-monomer interactions upon membrane binding by creating new contacts. Notably, residues at the interface are conserved in other members of the CDC family, suggesting a common mechanism for pore and pre-pore assembly.


Authors: Marshall, J.E., Faraj, B.H.A., Gingras, A.R., Lonnen, R., Sheikh, M.A., El-Mezgueldi, M., Moody, P.C.E., Andrew, P.W., Wallis, R.
The Crystal Structure of Pneumolysin at 2.0 A Resolution Reveals the Molecular Packing of the Pre-pore Complex.,Marshall JE, Faraj BH, Gingras AR, Lonnen R, Sheikh MA, El-Mezgueldi M, Moody PC, Andrew PW, Wallis R Sci Rep. 2015 Sep 3;5:13293. doi: 10.1038/srep13293. PMID:26333773<ref>PMID:26333773</ref>


Description: Structure of the membrane-binding domain of pneumolysin
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Faraj, B.H.A]]
<div class="pdbe-citations 5cr8" style="background-color:#fffaf0;"></div>
[[Category: Andrew, P.W]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Andrew, P W]]
[[Category: El-Mezgueldi, M]]
[[Category: Faraj, B H.A]]
[[Category: Gingras, A R]]
[[Category: Lonnen, R]]
[[Category: Lonnen, R]]
[[Category: Sheikh, M.A]]
[[Category: Marshall, J E]]
[[Category: Marshall, J.E]]
[[Category: Moody, P C.E]]
[[Category: Gingras, A.R]]
[[Category: Sheikh, M A]]
[[Category: Moody, P.C.E]]
[[Category: Wallis, R]]
[[Category: Wallis, R]]
[[Category: El-Mezgueldi, M]]
[[Category: Cholesterol-dependent cytolysin]]
[[Category: Hydrolase]]
[[Category: Toxin]]
[[Category: Virulence factor]]
Retrieved from "https://proteopedia.org/5cr8"