IspG: Difference between revisions
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Michal Harel (talk | contribs) New page: <StructureSection load='4g9p' size='340' side='right' caption='IspG containing 4Fe4S cluster complex with MEcPP (PDB entry 4g9p)' scene=''> == Function == IspG is a 4Fe4S protein w... |
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<StructureSection load='4g9p' size='340' side='right' caption='IspG containing 4Fe4S cluster complex with MEcPP, MES, K+ (purple) and Cl- (green) ions (PDB entry [[4g9p]])' scene=''> | |||
<StructureSection load='4g9p' size='340' side='right' caption='IspG containing 4Fe4S cluster complex with MEcPP (PDB entry [[4g9p]])' scene=''> | |||
== Function == | == Function == | ||
IspG is a 4Fe4S protein which serves as the penultimate enzyme of the non-mevalonate pathway for the biosynthesis os isoprenoid precursors like isopentenyl diphosphate and dimethylallyl <ref>PMID:20932974</ref>. It catalyzes the ring opening of 2C-methyl-D-erythritol 2,4-cyclodiphosphate (MEcPP) producing (E)-1-hydroxyl-2-methyl-but-2-enyl-4-diphosphate (HMBPP). | '''IspG''' is a 4Fe4S protein which serves as the penultimate enzyme of the non-mevalonate pathway for the biosynthesis os isoprenoid precursors like isopentenyl diphosphate and dimethylallyl <ref>PMID:20932974</ref>. It catalyzes the ring opening of 2C-methyl-D-erythritol 2,4-cyclodiphosphate (MEcPP) producing (E)-1-hydroxyl-2-methyl-but-2-enyl-4-diphosphate (HMBPP). | ||
== Relevance == | == Relevance == | ||
The non-mevlonate enzymes are a target for tuberculostatic and antimalarial drugs. | The non-mevlonate enzymes are a target for tuberculostatic and antimalarial drugs<ref>PMID:22586085</ref>. | ||
== Structural highlights == | == Structural highlights == | ||
The substrate MEcPP is located in the cavity between the TIM-barrel and the lid domain of IpsG. It binds covalently to the 4Fe4S cluster and its PP moiety binds to 2 Arg sidechains<ref>PMID:22967895</ref>. | |||
</StructureSection> | </StructureSection> | ||
== 3D Structures of IspG == | == 3D Structures of IspG == | ||
Revision as of 11:29, 28 November 2017
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3D Structures of IspG
Updated on 28-November-2017
3noy – IspG – Aquifex aeolicus
4mwa – IspG – Bacillus anthracis
2y0f – TtIspG – Thermus thermophilus
4s38, 4g9p – TtIspG + MEcPP
4s39, 4s23 – TtIspG + HMBPP
4s3a, 4s3b, 4s3c – TtIspG + intermediate
4s3c – TtIspG + PPi
4s3e, 4s3f – TtIspG + inhibitor
