5xxm: Difference between revisions
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==Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone== | |||
<StructureSection load='5xxm' size='340' side='right' caption='[[5xxm]], [[Resolution|resolution]] 1.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5xxm]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XXM FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LGC:(3S,4R,5R,6S)-3,4,5-TRIHYDROXY-6-(HYDROXYMETHYL)TETRAHYDRO-2H-PYRAN-2-ONE'>LGC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xxm OCA], [http://pdbe.org/5xxm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xxm RCSB], [http://www.ebi.ac.uk/pdbsum/5xxm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xxm ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
BT_3567 protein, a putative beta-glucosidase from Bacteroides thetaiotaomicron, exhibits higher activity toward Sop3-5 (Sopn , n: degree of polymerization of beta-1,2-glucooligosaccharides) than toward Sop2 , unlike a known beta-glucosidase from Listeria innocua which predominantly prefers Sop2 . In the complex structure determined by soaking of a D286N mutant crystal with Sop4 , a Sop3 moiety was observed at subsites -1 to +2. The glucose moiety at subsite +2 forms a hydrogen bond with Asn81, which is replaced with Gly in the L. innocua beta-glucosidase. The Km values of the N81G mutant for Sop3-5 are much higher than those of the wild-type, suggesting that Asn81 contributes to the binding to substrates longer than Sop3 . | |||
Function and structure relationships of a beta-1,2-glucooligosaccharide-degrading beta-glucosidase.,Ishiguro R, Tanaka N, Abe K, Nakajima M, Maeda T, Miyanaga A, Takahashi Y, Sugimoto N, Nakai H, Taguchi H FEBS Lett. 2017 Dec;591(23):3926-3936. doi: 10.1002/1873-3468.12911. Epub 2017, Nov 24. PMID:29131329<ref>PMID:29131329</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5xxm" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Abe, K]] | |||
[[Category: Ishiguro, R]] | |||
[[Category: Maeda, T]] | |||
[[Category: Miyanaga, A]] | |||
[[Category: Nakai, H]] | [[Category: Nakai, H]] | ||
[[Category: Nakajima, M]] | [[Category: Nakajima, M]] | ||
[[Category: Sugimoto, N]] | |||
[[Category: Taguchi, H]] | [[Category: Taguchi, H]] | ||
[[Category: Takahash, Y]] | [[Category: Takahash, Y]] | ||
[[Category: Tanaka, N]] | [[Category: Tanaka, N]] | ||
[[Category: | [[Category: Beta-glucosidase]] | ||
[[Category: | [[Category: Glycoside hydrolase family 3]] | ||
[[Category: | [[Category: Hydrolase]] | ||
