5upb

Swit_4259, an Acetoacetate Decarboxylase-like Enzyme from Sphingomonas wittichii RW1

spinqualitylabelsall models 5upb, resolution 1.91Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5upb is a 4 chain structure with sequence from Sphww. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: Swit_4259 (SPHWW) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The Gram-negative bacterium Sphingomonas wittichii RW1 is notable for its ability to metabolize a variety of aromatic hydrocarbons. Not surprisingly, the S. wittichii genome contains a number of putative aromatic hydrocarbon-degrading gene clusters. One of these includes an enzyme of unknown function, Swit_4259, which belongs to the acetoacetate decarboxylase-like superfamily (ADCSF). Here, it is reported that Swit_4259 is a small (28.8 kDa) tetrameric ADCSF enzyme that, unlike the prototypical members of the superfamily, does not have acetoacetate decarboxylase activity. Structural characterization shows that the tertiary structure of Swit_4259 is nearly identical to that of the true decarboxylases, but there are important differences in the fine structure of the Swit_4259 active site that lead to a divergence in function. In addition, it is shown that while it is a poor substrate, Swit_4259 can catalyze the hydration of 2-oxo-hex-3-enedioate to yield 2-oxo-4-hydroxyhexanedioate. It is also demonstrated that Swit_4259 has pyruvate aldolase-dehydratase activity, a feature that is common to all of the family V ADCSF enzymes studied to date. The enzymatic activity, together with the genomic context, suggests that Swit_4259 may be a hydratase with a role in the metabolism of an as-yet-unknown hydrocarbon. These data have implications for engineering bioremediation pathways to degrade specific pollutants, as well as structure-function relationships within the ADCSF in general. Swit_4259, an acetoacetate decarboxylase-like enzyme from Sphingomonas wittichii RW1.,Mydy LS, Mashhadi Z, Knight TW, Fenske T, Hagemann T, Hoppe RW, Han L, Miller TR, Schwabacher AW, Silvaggi NR Acta Crystallogr F Struct Biol Commun. 2017 Dec 1;73(Pt 12):672-681. doi:, 10.1107/S2053230X17015862. Epub 2017 Nov 14. PMID:29199988 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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