4b5y

X-ray structure of the cyan fluorescent protein mTurquoise-GL (K206A mutant) in space group C222(1)

spinqualitylabelsall models 4b5y, resolution 1.45Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4b5y is a 1 chain structure with sequence from Aeqvi. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: SWG Related: 1b9c, 1bfp, 1c4f, 1cv7, 1ema, 1emb, 1emc, 1eme, 1emf, 1emg, 1emk, 1eml, 1emm, 1f09, 1f0b, 1gfl, 1h6r, 1hcj, 1huy, 1jby, 1jbz, 1jc0, 1jc1, 1kp5, 1kyp, 1kyr, 1kys, 1myw, 1q4a, 1q4b, 1q4c, 1q4d, 1q4e, 1q73, 1qxt, 1qy3, 1qyf, 1qyo, 1qyq, 1rm9, 1rmm, 1rmo, 1rmp, 1rrx, 1w7s, 1w7t, 1w7u, 1yfp, 1yhg, 1yhh, 1yhi, 1yj2, 1yjf, 1z1p, 1z1q, 2ah8, 2aha, 2b3p, 2b3q, 2emd, 2emn, 2emo, 2fwq, 2fzu, 2wsn, 2wso, 2wur, 2y0g, 2ydz, 2ye0, 2ye1, 2yfp, 3ztf, 4anj, 4ar7, 4as8 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Publication Abstract from PubMed Optimization of autofluorescent proteins by intensity-based screening of bacteria does not necessarily identify the brightest variant for eukaryotes. We report a strategy to screen excited state lifetimes, which identified cyan fluorescent proteins with long fluorescence lifetimes (>3.7 ns) and high quantum yields (>0.8). One variant, mTurquoise, was 1.5-fold brighter than mCerulean in mammalian cells and decayed mono-exponentially, making it an excellent fluorescence resonance energy transfer (FRET) donor. Bright cyan fluorescent protein variants identified by fluorescence lifetime screening.,Goedhart J, van Weeren L, Hink MA, Vischer NO, Jalink K, Gadella TW Jr Nat Methods. 2010 Feb;7(2):137-9. Epub 2010 Jan 17. PMID:20081836 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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