5uj1: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 5uj1 is ~~ON HOLD until Jan 17 2019~~

==Crystal structure of Mycobacterium tuberculosis Topoisomerase I at 2.15A resolution limit==

~~Authors~~: ~~Cao~~, N., ~~Tan~~, K., ~~Tse-Dinh~~, Y.C.

== Structural highlights ==

<table><tr><td colspan='2'>[[5uj1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UJ1 OCA]. For a guided tour on the structure components use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UJ1 FirstGlance].

~~Description~~: ~~Crystal structure~~ of ~~Mycobacterium tuberculosis Topoisomerase I~~ at 2.~~15A resolution limit~~

</td></tr><tr id='ligand'><td class="sblockLbl">[[Ligand|Ligands:]]</td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

[[Category: ~~Unreleased Structures~~]]

<tr id='related'><td class="sblockLbl">[[Related_structure|Related:]]</td><td class="sblockDat">[[5d5h|5d5h]], [[5ujy|5ujy]], [[5ukt|5ukt]]</td></tr>

[[Category: ~~Tse-Dinh~~, ~~Y.C~~]]

<tr id='activity'><td class="sblockLbl">Activity:</td><td class="sblockDat">[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </td></tr>

<tr id='resources'><td class="sblockLbl">Resources:</td><td class="sblockDat">[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uj1 OCA], [http://pdbe.org/5uj1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uj1 RCSB], [http://www.ebi.ac.uk/pdbsum/5uj1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uj1 ProSAT]</td></tr>

</table>

== Function ==

[[http://www.uniprot.org/uniprot/A0A0E8VY41_MYCTX A0A0E8VY41_MYCTX]] Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.[HAMAP-Rule:MF_00952]

__TOC__

</StructureSection>

[[Category: DNA topoisomerase]]

[[Category: Cao, N]]

[[Category: Tan, K]]

[[Category: ~~Cao~~, N]]

[[Category: Tse-Dinh, Y C]]

[[Category: Isomerase]]

[[Category: Mycobacterium tuberculosis]]

[[Category: Topoisomerase i]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5uj1 is ON HOLD  until Jan 17 2019
+==Crystal structure of Mycobacterium tuberculosis Topoisomerase I at 2.15A resolution limit==
+<StructureSection load='5uj1' size='340' side='right' caption='[[5uj1]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
-Authors: Cao, N., Tan, K., Tse-Dinh, Y.C.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5uj1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UJ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UJ1 FirstGlance]. <br>
-Description: Crystal structure of Mycobacterium tuberculosis Topoisomerase I at 2.15A resolution limit
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-[[Category: Unreleased Structures]]
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5d5h|5d5h]], [[5ujy|5ujy]], [[5ukt|5ukt]]</td></tr>
-[[Category: Tse-Dinh, Y.C]]
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uj1 OCA], [http://pdbe.org/5uj1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uj1 RCSB], [http://www.ebi.ac.uk/pdbsum/5uj1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uj1 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/A0A0E8VY41_MYCTX A0A0E8VY41_MYCTX]] Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.[HAMAP-Rule:MF_00952]
+__TOC__
+</StructureSection>
+[[Category: DNA topoisomerase]]
+[[Category: Cao, N]]
 [[Category: Tan, K]]
-[[Category: Cao, N]]
+[[Category: Tse-Dinh, Y C]]
+[[Category: Isomerase]]
+[[Category: Mycobacterium tuberculosis]]
+[[Category: Topoisomerase i]]