3rsj

Structure of HCRF in complex with Ganglioside GD1a

spinqualitylabelsall models 3rsj, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3rsj is a 4 chain structure with sequence from "bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GAL, NGA, SIA Related: 3fuq Gene: bont/f, Botulinum Neurotoxin ("Bacillus botulinus" van Ermengem 1896) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Botulinum neurotoxins (BoNTs) and tetanus neurotoxin (TeNT) are the causative agents of the paralytic diseases botulism and tetanus, respectively. The potency of the clostridial neurotoxins (CNTs) relies primarily on their highly specific binding to nerve terminals and cleavage of SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) proteins. While individual CNTs utilize distinct proteins for entry, they share common ganglioside co-receptors. Here we report the crystal structure of the BoNT/F receptor binding domain in complex with the sugar moiety of ganglioside GD1a. GD1a binds in a shallow groove formed by a conserved peptide motif E...H...SXWY...G, with additional stabilizing interactions provided by two arginine residues. Comparative analysis of BoNT/F with other CNTs revealed several differences in the interactions of each toxin with ganglioside. Notably, exchange of BoNT/F His1241 with the corresponding lysine residue of BoNT/E resulted in increased affinity towards GD1a and conferred the ability to bind ganglioside GM1a. Conversely, BoNT/E was not able to bind GM1a, demonstrating a discreet mechanism of ganglioside recognition. These findings provide a structural basis for ganglioside binding among the clostridial neurotoxins and show that individual toxins utilize unique ganglioside recognition strategies. Unique ganglioside recognition strategies for clostridial neurotoxins.,Benson MA, Fu Z, Kim JJ, Baldwin MR J Biol Chem. 2011 Aug 17. PMID:21849494 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Botulinum neurotoxin

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