4oua

Coexistent single-crystal structure of latent and active mushroom tyrosinase (abPPO4) mediated by a hexatungstotellurate(VI)

spinqualitylabelsall models 4oua, resolution 2.76Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4oua is a 2 chain structure with sequence from Agaricus bisporus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CU1, NA, TEW, TRS NonStd Res: SAC Activity: Tyrosinase, with EC number 1.14.18.1 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Tyrosinases, bifunctional metalloenzymes, catalyze the oxidation of monophenols and o-diphenols to o-quinones, the precursor compounds of the brown-coloured pigment melanin. In eukaryotic organisms, tyrosinases are expressed as latent zymogens that have to be proteolytically cleaved in order to form highly active enzymes. This activation mechanism, known as the tyrosinase maturation process, has scientific and industrial significance with respect to biochemical and technical applications of the enzyme. Here, not only the first crystal structure of the mushroom tyrosinase abPPO4 is presented in its active form (Ser2-Ser383) and in its 21 kDa heavier latent form (Ser2-Thr545), but furthermore the simultaneous presence of both forms within one single-crystal structure is shown. This allows for a simple approach to investigate the transition between these two forms. Isoform abPPO4 was isolated and extensively purified from the natural source (Agaricus bisporus), which contains a total of six polyphenol oxidases (PPOs). The enzyme formed crystals (diffracting to a resolution of 2.76 A) owing to the employment of the 6-tungstotellurate(VI) salt (Na6[TeW6O24].22H2O) as a cocrystallization agent. Two of these disc-shaped Anderson-type polyoxoanions [TeW6O24](6-) separate two asymmetric units comprising one crystallographic heterodimer of abPPO4, thus resulting in very interesting crystal packing. Latent and active abPPO4 mushroom tyrosinase cocrystallized with hexatungstotellurate(VI) in a single crystal.,Mauracher SG, Molitor C, Al-Oweini R, Kortz U, Rompel A Acta Crystallogr D Biol Crystallogr. 2014 Sep 1;70(Pt 9):2301-15. doi:, 10.1107/S1399004714013777. Epub 2014 Aug 29. PMID:25195745 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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