5z7i: Difference between revisions
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==Caulobacter crescentus GcrA DNA-binding domain(DBD)in complex with unmethylated dsDNA== | |||
<StructureSection load='5z7i' size='340' side='right' caption='[[5z7i]], [[Resolution|resolution]] 1.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5z7i]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z7I FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BU3:(R,R)-2,3-BUTANEDIOL'>BU3</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z7i OCA], [http://pdbe.org/5z7i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z7i RCSB], [http://www.ebi.ac.uk/pdbsum/5z7i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z7i ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Canonical bacterial transcription activators bind to non-transcribed promoter elements to increase transcription of their target genes. Here we report crystal structures of binary complexes comprising domains of Caulobacter crescentus GcrA, a noncanonical bacterial transcription factor, that support a novel mechanism for transcription activation through the preferential binding of methylated cis-regulatory elements and the promotion of open complex formation through an interaction with region 2 of the principal sigma factor, sigma70. We present crystal structures of the C-terminal, sigma factor-interacting domain (GcrA-SID) in complex with domain 2 of sigma70 (sigma702), and the N-terminal, DNA-binding domain (GcrA-DBD) in complex with methylated double-stranded DNA (dsDNA). The structures reveal interactions essential for transcription activation and DNA recognition by GcrA. These structures, along with mutational analyses, support a mechanism of transcription activation in which GcrA associates with RNA polymerase (RNAP) prior to promoter binding through GcrA-SID, arming RNAP with a flexible GcrA-DBD. The RNAP-GcrA complex then binds and activates target promoters harboring a methylated GcrA binding site either upstream or downstream of the transcription start site. | |||
Structural insights into the unique mechanism of transcription activation by Caulobacter crescentus GcrA.,Wu X, Haakonsen DL, Sanderlin AG, Liu YJ, Shen L, Zhuang N, Laub MT, Zhang Y Nucleic Acids Res. 2018 Mar 5. pii: 4920855. doi: 10.1093/nar/gky161. PMID:29514271<ref>PMID:29514271</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5z7i" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Wu, X]] | |||
[[Category: Zhang, Y]] | [[Category: Zhang, Y]] | ||
[[Category: | [[Category: Caulobacter crescentus]] | ||
[[Category: Dna binding protein]] | |||
[[Category: Dna binding protein-dna complex]] | |||
[[Category: Dna-binding]] | |||
[[Category: Gcra]] | |||
[[Category: Transcription factor]] | |||
Revision as of 07:36, 21 March 2018
Caulobacter crescentus GcrA DNA-binding domain(DBD)in complex with unmethylated dsDNA
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