5wb0: Difference between revisions
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wb0 OCA], [http://pdbe.org/5wb0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wb0 RCSB], [http://www.ebi.ac.uk/pdbsum/5wb0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wb0 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wb0 OCA], [http://pdbe.org/5wb0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wb0 RCSB], [http://www.ebi.ac.uk/pdbsum/5wb0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wb0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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== Publication Abstract from PubMed == | |||
Human metapneumovirus (hMPV) is a frequent cause of bronchiolitis in young children. Its F glycoprotein mediates virus-cell membrane fusion and is the primary target of neutralizing antibodies. The inability to produce recombinant hMPV F glycoprotein in the metastable pre-fusion conformation has hindered structural and immunological studies. Here, we engineer a pre-fusion-stabilized hMPV F ectodomain and determine its crystal structure to 2.6 A resolution. This structure reveals molecular determinants of strain-dependent acid-induced fusion, as well as insights into refolding from pre- to post-fusion conformations. A dense glycan shield at the apex of pre-fusion hMPV F suggests that antibodies against this site may not be elicited by host immune responses, which is confirmed by depletion studies of human immunoglobulins and by mouse immunizations. This is a major difference with pre-fusion F from human respiratory syncytial virus (hRSV), and collectively our results should facilitate development of effective hMPV vaccine candidates. | |||
Structure and immunogenicity of pre-fusion-stabilized human metapneumovirus F glycoprotein.,Battles MB, Mas V, Olmedillas E, Cano O, Vazquez M, Rodriguez L, Melero JA, McLellan JS Nat Commun. 2017 Nov 16;8(1):1528. doi: 10.1038/s41467-017-01708-9. PMID:29142300<ref>PMID:29142300</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
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== References == | |||
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