User:Mark Macbeth/Sandbox2: Difference between revisions
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<StructureSection load='1b7f' size='350' side='right' caption='Sex-Lethal protein' scene=''> | |||
== Background == | == Background == | ||
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The Sxl RNA splicing targets encode for the transformer (''tra'') and the male-sex lethal (''msl-2'') proteins. ''Tra'' is a splicing activator for the female developmental pathway, and ''msl-2'' modulates [http://en.wikipedia.org/wiki/X_chromosome X chromosome] application in male fruit flies. The mechanism for how Sxl targets these pathways differs slightly. In both mechanisms, Sxl occupies the 3' splice site and prevents [http://en.wikipedia.org/wiki/U2AF2 U2AF] from binding. This causes the U2AF splicing factor to bind at a downstream splice site encoding proteins in the female developmental pathway. In ''msl-2'' targeting, Sxl also blocks the binding of another regulatory splicing factor, Rox8, and the [http://en.wikipedia.org/wiki/SnRNP U1 snRNP] at the 5’ splice site<ref name="Penalva"/>. Sxl can also control its own splicing pattern to conserve female expression. It does so by binding to [http://en.wikipedia.org/wiki/Exon Exon] 3 of its own RNA and creating an RNP complex to eliminate this exon. After removal of Exon 3, Sxl becomes active and female expression is maintained. | The Sxl RNA splicing targets encode for the transformer (''tra'') and the male-sex lethal (''msl-2'') proteins. ''Tra'' is a splicing activator for the female developmental pathway, and ''msl-2'' modulates [http://en.wikipedia.org/wiki/X_chromosome X chromosome] application in male fruit flies. The mechanism for how Sxl targets these pathways differs slightly. In both mechanisms, Sxl occupies the 3' splice site and prevents [http://en.wikipedia.org/wiki/U2AF2 U2AF] from binding. This causes the U2AF splicing factor to bind at a downstream splice site encoding proteins in the female developmental pathway. In ''msl-2'' targeting, Sxl also blocks the binding of another regulatory splicing factor, Rox8, and the [http://en.wikipedia.org/wiki/SnRNP U1 snRNP] at the 5’ splice site<ref name="Penalva"/>. Sxl can also control its own splicing pattern to conserve female expression. It does so by binding to [http://en.wikipedia.org/wiki/Exon Exon] 3 of its own RNA and creating an RNP complex to eliminate this exon. After removal of Exon 3, Sxl becomes active and female expression is maintained. | ||
== Structure == | == Structure == | ||
