2jeu: Difference between revisions
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jeu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jeu OCA], [http://www.ebi.ac.uk/pdbsum/2jeu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jeu RCSB]</span> | |||
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[[Category: viral transcription factor]] | [[Category: viral transcription factor]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:57:12 2008'' | ||
Revision as of 00:57, 31 March 2008
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| 2jeu, resolution 2.80Å | |||||||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
TRANSCRIPTION ACTIVATOR STRUCTURE REVEALS REDOX CONTROL OF A REPLICATION INITIATION REACTION
Overview
Redox changes are one of the factors that influence cell-cycle progression and that control the processes of cellular proliferation, differentiation, senescence and apoptosis. Proteins regulated through redox-sensitive cysteines have been characterized but specific 'sulphydryl switches' in replication proteins remain to be identified. In bovine papillomavirus type-1, DNA replication begins when the viral transcription factor E2 recruits the viral initiator protein E1 to the origin of DNA replication (ori). Here we show that a novel dimerization interface in the E2 transcription activation domain is stabilized by a disulphide bond. Oxidative cross-linking via Cys57 sequesters the interaction surface between E1 and E2, preventing pre-initiation and replication initiation complex formation. Our data demonstrate that as well as a mechanism for regulating DNA binding, redox reactions can control replication by modulating the tertiary structure of critical protein factors using a specific redox sensor.
About this Structure
2JEU is a Single protein structure of sequence from Bovine papillomavirus type 1. Full crystallographic information is available from OCA.
Reference
Transcription activator structure reveals redox control of a replication initiation reaction., Sanders CM, Sizov D, Seavers PR, Ortiz-Lombardia M, Antson AA, Nucleic Acids Res. 2007;35(10):3504-15. Epub 2007 May 3. PMID:17478495
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Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Bovine papillomavirus type 1
- Single protein
- Antson, A A.
- Ortiz-Lombardia, M.
- Sanders, C M.
- Seavers, P R.
- Sizov, D.
- Activator
- Bovine papillomavirus
- Dna replication
- Dna-binding
- E1
- E2
- Early protein
- Nuclear protein
- Oxidation
- Phosphorylation
- Redox control
- Replication initiation
- Repressor
- Transcription
- Transcription regulation
- Viral transcription factor
