6ck5: Difference between revisions
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The | ==PRPP riboswitch from T. mathranii bound to PRPP== | ||
<StructureSection load='6ck5' size='340' side='right' caption='[[6ck5]], [[Resolution|resolution]] 2.49Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6ck5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CK5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CK5 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRP:ALPHA-PHOSPHORIBOSYLPYROPHOSPHORIC+ACID'>PRP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ck5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ck5 OCA], [http://pdbe.org/6ck5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ck5 RCSB], [http://www.ebi.ac.uk/pdbsum/6ck5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ck5 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Two classes of riboswitches related to the ykkC guanidine-I riboswitch bind phosphoribosyl pyrophosphate (PRPP) and guanosine tetraphosphate (ppGpp). Here we report the co-crystal structure of the PRPP aptamer and its ligand. We also report the structure of the G96A point mutant that prefers ppGpp over PRPP with a dramatic 40,000-fold switch in specificity. The ends of the aptamer form a helix that is not present in the guanidine aptamer and is involved in the expression platform. In the mutant, the base of ppGpp replaces G96 in three-dimensional space. This disrupts the S-turn, which is a primary structural feature of the ykkC RNA motif. These dramatic differences in ligand specificity are achieved with minimal mutations. ykkC aptamers are therefore a prime example of an RNA fold with a rugged fitness landscape. The ease with which the ykkC aptamer acquires new specificity represents a striking case of evolvability in RNA. | |||
Structures of two aptamers with differing ligand specificity reveal ruggedness in the functional landscape of RNA.,Knappenberger AJ, Reiss CW, Strobel SA Elife. 2018 Jun 7;7. pii: 36381. doi: 10.7554/eLife.36381. PMID:29877798<ref>PMID:29877798</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6ck5" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Knappenberger, A J]] | |||
[[Category: Reiss, C W]] | |||
[[Category: Strobel, S A]] | |||
[[Category: Prpp]] | |||
[[Category: Riboswitch]] | |||
[[Category: Rna]] | |||
[[Category: Ykkc]] | |||
Revision as of 05:50, 20 June 2018
PRPP riboswitch from T. mathranii bound to PRPP
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