5wez: Difference between revisions
From Proteopedia
Jump to navigationJump to search
m Protected "5wez" [edit=sysop:move=sysop] |
No edit summary |
||
| Line 1: | Line 1: | ||
The | ==Structure of the Tir-CesT effector-chaperone complex== | ||
<StructureSection load='5wez' size='340' side='right' caption='[[5wez]], [[Resolution|resolution]] 2.74Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5wez]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WEZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WEZ FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wez OCA], [http://pdbe.org/5wez PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wez RCSB], [http://www.ebi.ac.uk/pdbsum/5wez PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wez ProSAT]</span></td></tr> | |||
[[Category: | </table> | ||
== Function == | |||
[[http://www.uniprot.org/uniprot/CEST_ECO27 CEST_ECO27]] Chaperone for the type III secretion of Tir. Probably stabilizes the protein, prevents inappropriate protein-proteininteractions and aids in secretion. [[http://www.uniprot.org/uniprot/TIR_ECO27 TIR_ECO27]] Multifunctional protein that is required for efficient pedestal formation in host epithelial cells during infection. The extracellular region acts as a receptor for bacterial intimin, allowing the bacterium to attach tightly to the host-cell surface. Simultaneously, the intracellular region initiates a signaling cascade in the host cell, which leads to actin polymerization and formation of actin pedestals at the sites of bacterial adhesion. In strain E2348/69, acts mainly via the host adaptor proteins NCK1 and NCK2. Once clustered and phosphorylated at Tyr-474, Tir binds to NCK proteins, which in turn bind and activate host WASL/N-WASP, leading to actin polymerization. Can also trigger an inefficient, NCK-independent pedestal formation. This pathway involves phosphorylation of Tyr-454 and probably a putative host adaptor. Acts also via direct binding to the host cytoskeletal protein alpha-actinin in a NCK- and phosphotyrosine-independent manner. This interaction may stabilize the pedestal, but is not essential for its formation.<ref>PMID:10096089</ref> <ref>PMID:14757753</ref> <ref>PMID:14764108</ref> <ref>PMID:15813734</ref> <ref>PMID:17521329</ref> <ref>PMID:9390560</ref> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Coombes, B K]] | |||
[[Category: Little, D J]] | |||
[[Category: Chaperone]] | |||
[[Category: Complex]] | |||
[[Category: Effector]] | |||
[[Category: Secretion]] | |||
[[Category: Translocation]] | |||
Revision as of 05:36, 11 July 2018
Structure of the Tir-CesT effector-chaperone complex
| ||||||||||||
