6gg9: Difference between revisions
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==Crystal structures of a short blue light photoreceptor protein PpSB1-LOV mutant (dark state) - R61H/R66I== | |||
<StructureSection load='6gg9' size='340' side='right' caption='[[6gg9]], [[Resolution|resolution]] 2.04Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6gg9]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GG9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GG9 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gg9 OCA], [http://pdbe.org/6gg9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gg9 RCSB], [http://www.ebi.ac.uk/pdbsum/6gg9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gg9 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Light, oxygen, voltage (LOV) photoreceptors consist of conserved photo-responsive domains in bacteria, archaea, plants and fungi, and detect blue-light via a flavin cofactor. We investigated the blue-light induced conformational transition of the dimeric photoreceptor PpSB1-LOV-R66I from Pseudomonas putida in solution by using small-angle X-ray scattering (SAXS). SAXS experiments of the fully populated light- and dark-states under steady-state conditions revealed significant structural differences between the two states that are in agreement with the known structures determined by crystallography. We followed the transition from the light- to the dark-state by using SAXS measurements in real-time. A two-state model based on the light- and dark-state conformations could describe the measured time-course SAXS data with a relaxation time tauREC of ~ 34 to 35 min being larger than the recovery time found with UV/vis spectroscopy. Unlike the flavin chromophore-based UV/vis method that is sensitive to the local chromophore environment in flavoproteins, SAXS-based assay depends on protein conformational changes and provides with an alternative to measure the recovery kinetics. | |||
Small-angle X-ray scattering study of the kinetics of light-dark transition in a LOV protein.,Rollen K, Granzin J, Batra-Safferling R, Stadler AM PLoS One. 2018 Jul 16;13(7):e0200746. doi: 10.1371/journal.pone.0200746., eCollection 2018. PMID:30011332<ref>PMID:30011332</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6gg9" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Batra-Safferling, R]] | |||
[[Category: Granzin, J]] | |||
[[Category: Roellen, K]] | |||
[[Category: Blue light photoreceptor]] | |||
[[Category: Sensory box protein]] | |||
[[Category: Signaling protein]] | |||
Latest revision as of 07:23, 25 July 2018
Crystal structures of a short blue light photoreceptor protein PpSB1-LOV mutant (dark state) - R61H/R66I
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