Mediator: Difference between revisions
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== Mediator == | == Mediator == | ||
The '''mediator''', found in the yeast ''S. cerevisiae'', connects repressors and activators bound to regulatory DNA with RNA polymerase II (Pol II). As a result, the mediator is an important regulator of eukaryotic transcription.<ref>PMID: 21725323 </ref> Also, mediator is currently being studied because of its ability and role to regulate gene expression.<ref>PMID: 20299225 </ref> Mediator is in the form of three main modules, which are the tail, arm, and head. The <scene name='Sandbox_Reserved_387/Head_module/1'>head module</scene> of the mediator is split into three domains that go through significant conformational changes, and it interacts with the Rpb7 and Rpb4 subunits of Pol II and TATA binding protein subunit of transcription factor TFIID.<ref name="nature">PMID: 21725323 </ref> | The '''mediator'''or '''mediator of RNA polymerase II transcription subunit''', found in the yeast ''S. cerevisiae'', connects repressors and activators bound to regulatory DNA with RNA polymerase II (Pol II). As a result, the mediator is an important regulator of eukaryotic transcription.<ref>PMID: 21725323 </ref> Also, mediator is currently being studied because of its ability and role to regulate gene expression.<ref>PMID: 20299225 </ref> Mediator is in the form of three main modules, which are the tail, arm, and head. The <scene name='Sandbox_Reserved_387/Head_module/1'>head module</scene> of the mediator is split into three domains that go through significant conformational changes, and it interacts with the Rpb7 and Rpb4 subunits of Pol II and TATA binding protein subunit of transcription factor TFIID.<ref name="nature">PMID: 21725323 </ref> | ||
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==3D structures of mediator== | ==3D structures of mediator== | ||
[[3rj1]] – Med6 + Med8 + Med11 + Med17 + Med18 + Med20 + Med22 - | [[3rj1]] – yMed6 + Med8 + Med11 + Med17 + Med18 + Med20 + Med22 - yeast<br /> | ||
[[1yke]], [[1ykh]] – | [[5u0s]] – fyMed4 + Med6 + Med7 + Med8 + Med9 + Med10 + Med11 + Med14 + Med17 + Med18 + Med19 + Med20 + Med21 + Med22 + Med27 + Med31 + RPB1 + RPB2 + RPB3 + RPB4 + RPB5 + RPB6 + RPB7 + RPB8 + RPB9 + RPB10 + RPB11 + RPB12 – fission yeast – Cryo EM<br /> | ||
[[3fbi]], [[3fbn]] – | [[5u0s]] – fyMed4 + Med6 + Med7 + Med8 + Med9 + Med10 + Med11 + Med14 + Med17 + Med18 + Med19 + Med20 + Med21 + Med22 + Med27 + Med31 – Cryo EM<br /> | ||
[[3c0t]] - | [[5n9j]] – fyMed4 + Med6 + Med7 + Med8 + Med9 + Med10 + Med11 + Med14 + Med17 + Med18 + Med19 + Med20 + Med21 + Med22 + Med31 <br /> | ||
[[2hzs]] – | [[5sva]] – fyMed4 + Med6 + Med7 + Med8 + Med9 + Med11 + Med17 + Med18 + Med20 + Med21 + Med22 + Med31 + pre-initiation complex – Cryo EM<br /> | ||
[[3r84]] - | [[4v1o]] – yMed8 + Med11 + Med18 + Med20 + Med22 + pre-initiation complex – Cryo EM<br /> | ||
[[2lpb]] - | [[1yke]], [[1ykh]] – yMed7 + Med21 <br /> | ||
[[2hzm]] – | [[3fbi]], [[3fbn]] – yMed7 N terminal + Med31<br /> | ||
[[2l23]], [[2l6u]], [[2ky6]], [[2xnf]] – | [[3c0t]] - fyMed8 C terminal + Med18<br /> | ||
[[2hzs]] – yMed8 C terminal + Med18 + Med20<br /> | |||
[[3r84]] - yMed11 + Med22 (mutant)<br /> | |||
[[6aly]] – yMed 15 ABD2 residues<br /> | |||
[[2lpb]] - yMed15 + general control protein GCN4<br /> | |||
[[2hzm]] – yMed18 + Med20<br /> | |||
[[2l23]], [[2l6u]], [[2ky6]], [[2xnf]] – hMed25 activator-interacting domain - human - NMR<br /> | |||
[[5odd]] – hMed26 N terminal<br /> | |||
