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| Line 24: |
Line 24: |
| **[[1nec]], [[1kqd]] – EclNR – ''Enterobacter cloacae''<br /> | | **[[1nec]], [[1kqd]] – EclNR – ''Enterobacter cloacae''<br /> |
| **[[1ywq]], [[2i7h]] – NR – ''Bacillus cereus''<br /> | | **[[1ywq]], [[2i7h]] – NR – ''Bacillus cereus''<br /> |
| | **[[5hdj]] – NR – ''Bacillus megaterium''<br /> |
| **[[2b67]] – NR – ''Streptococcus pneumonia''<br /> | | **[[2b67]] – NR – ''Streptococcus pneumonia''<br /> |
| **[[3ek3]], [[3hoi]] – NR – ''Bacterioides fragilis''<br /> | | **[[3ek3]], [[3hoi]] – NR – ''Bacterioides fragilis''<br /> |
| Line 49: |
Line 50: |
| **[[4bn9]] – LlNR + nicotinic acid<br /> | | **[[4bn9]] – LlNR + nicotinic acid<br /> |
| **[[4bnb]] – LlNR + nitroquinoline<br /> | | **[[4bnb]] – LlNR + nitroquinoline<br /> |
| | |
| | *Nitrobenzoate nitroreductase |
| | |
| | **[[4z85]] – NR – ''Psudomonas fluorescens''<br /> |
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| |
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| *Nitroreductase binary complex | | *Nitroreductase binary complex |
| Line 59: |
Line 64: |
| **[[1oo6]], [[1oon]], [[1idt]] – EcNR + benzamide derivative<br /> | | **[[1oo6]], [[1oon]], [[1idt]] – EcNR + benzamide derivative<br /> |
| **[[1yki]] – EcNR + antibiotic<br /> | | **[[1yki]] – EcNR + antibiotic<br /> |
| **[[2wzw]] - MsNR + NADPH | | **[[2wzw]] - MsNR + NADPH<br /> |
| | **[[6czp]] – NR + FMN – ''Vibrio vulnificus''<br /> |
| }} | | }} |
| == References == | | == References == |
| <references/> | | <references/> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |
|
Function
Nitroreductase (NR) is involved in the reduction of nitrogen-containing compounds including those containing NO2 group. The NO2 group is reduced to hydroxylamine[1]. NR uses FMN as cofactor. Among NRs are the oxygen-insensitive NAD(P)H NR and NADH dehydrogenase.
Relevance
NR is of special interest due to its potential use as activator of prodrugs in cancer therapy[2].
Structural highlights
NR cofactor FMN is bound directly to one subunit while the ligand - nicotinic acid - interacts with both subunits. Water molecules shown as red spheres.
- ↑ Lovering AL, Hyde EI, Searle PF, White SA. The structure of Escherichia coli nitroreductase complexed with nicotinic acid: three crystal forms at 1.7 A, 1.8 A and 2.4 A resolution. J Mol Biol. 2001 May 25;309(1):203-13. PMID:11491290 doi:10.1006/jmbi.2001.4653
- ↑ Williams EM, Little RF, Mowday AM, Rich MH, Chan-Hyams JV, Copp JN, Smaill JB, Patterson AV, Ackerley DF. Nitroreductase gene-directed enzyme prodrug therapy: insights and advances toward clinical utility. Biochem J. 2015 Oct 15;471(2):131-53. doi: 10.1042/BJ20150650. PMID:26431849 doi:https://dx.doi.org/10.1042/BJ20150650
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3D structures of nitroreductase
Updated on 15-August-2018
{"openlevels":0}
- Nitroreductase
- 1f5v, 1oo5 – EcNR – Escherichia coli
- 3x21, 3x22 – EcNR (mutant)
- 3bm1, 1ds7 – EcNR minimal
- 3bm2 – EcNR minimal -FMN
- 1nec, 1kqd – EclNR – Enterobacter cloacae
- 1ywq, 2i7h – NR – Bacillus cereus
- 5hdj – NR – Bacillus megaterium
- 2b67 – NR – Streptococcus pneumonia
- 3ek3, 3hoi – NR – Bacterioides fragilis
- 3g14 – NR – Clostridium novyi
- 3h4o, 3koq - NR – Clostridium difficile
- 3gr3 – NR – Bartonella henselae
- 3hzn – NR – Salmonella typhimurium
- 3k6h – NR – Agrobacterium tumefaciens
- 2wzv, 2ymv – MsNR – Mycobacterium smegmatis
- 3of4 – NR FMN/FAD – Idiomarina loihiensis
- 3n2s – NR – Bacillus subtilis
- 3pxv – NR – Desulfitobacterium hafniense
- 3qdl – NR – Helicobacter pylori
- 3r5l, 3r5p - MtNR – Mycobacterium tuberculosis
- 3r5r, 3r5w – MtNR F420
- 3ge6 – NR – Exiguobacterium sibiricum
- 4dn2 – NR – Geobacter metallireducens
- 5ko7, 5ko8, 5krd – NR – Haliscomenobacter hydrossis
- Copper-induced nitroreductase
- 2wqf – LlNR – Lactococcus lactis
- 4bn6 – LlNR + chloramphenicol
- 4bn7, 4bn8 – LlNR + phenol derivative
- 4bn9 – LlNR + nicotinic acid
- 4bnb – LlNR + nitroquinoline
- Nitrobenzoate nitroreductase
- 4z85 – NR – Psudomonas fluorescens
- Nitroreductase binary complex
References
