2phf: Difference between revisions
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|PDB= 2phf |SIZE=350|CAPTION= <scene name='initialview01'>2phf</scene>, resolution 2.1Å | |PDB= 2phf |SIZE=350|CAPTION= <scene name='initialview01'>2phf</scene>, resolution 2.1Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> | |LIGAND= <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1q8p|1Q8P]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2phf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2phf OCA], [http://www.ebi.ac.uk/pdbsum/2phf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2phf RCSB]</span> | |||
}} | }} | ||
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[[Category: Loris, R.]] | [[Category: Loris, R.]] | ||
[[Category: Wyns, L.]] | [[Category: Wyns, L.]] | ||
[[Category: carbohydrate]] | [[Category: carbohydrate]] | ||
[[Category: high mannose]] | [[Category: high mannose]] | ||
[[Category: lectin]] | [[Category: lectin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:35:54 2008'' | ||
Revision as of 01:35, 31 March 2008
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| 2phf, resolution 2.1Å | |||||||||||||
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| Ligands: | BMA, CA, MAN, MN, PCA | ||||||||||||
| Related: | 1Q8P
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Pterocarpus angolensis lectin complexed with Man-6
Overview
The crystal structure of Pterocarpus angolensis seed lectin is presented in complex with a series of high mannose (Man) oligosaccharides ranging from Man-5 to Man-9. Despite that several of the nine Man residues of Man-9 have the potential to bind in the monosaccharide-binding site, all oligomannoses are bound in the same unique way, employing the tetrasaccharide sequence Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-. Isothermal titration calorimetry titration experiments using Man-5, Man-9, and the Man-9-containing glycoprotein soybean (Glycine max) agglutinin as ligands confirm the monovalence of Man-9 and show a 4-times higher affinity for Man-9 when it is presented to P. angolensis seed lectin in a glycoprotein context.
About this Structure
2PHF is a Single protein structure of sequence from Pterocarpus angolensis. Full crystallographic information is available from OCA.
Reference
How a plant lectin recognizes high mannose oligosaccharides., Garcia-Pino A, Buts L, Wyns L, Imberty A, Loris R, Plant Physiol. 2007 Aug;144(4):1733-41. Epub 2007 Jun 7. PMID:17556509
Page seeded by OCA on Mon Mar 31 04:35:54 2008
