2wzf

Legionella pneumophila glucosyltransferase crystal structure

spinqualitylabelsall models 2wzf, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2wzf is a 1 chain structure with sequence from Atcc 33152. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BGC, MN, UDP Related: 2wzg Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Legionnaires' disease is caused by a lethal colonization of alveolar macrophages with the Gram-negative bacterium Legionella pneumophila. LpGT (L. pneumophila glucosyltransferase; also known as Lgt1) has recently been identified as a virulence factor, shutting down protein synthesis in the human cell by specific glucosylation of EF1A (elongation factor 1A), using an unknown mode of substrate recognition and a retaining mechanism for glycosyl transfer. We have determined the crystal structure of LpGT in complex with substrates, revealing a GT-A fold with two unusual protruding domains. Through structure-guided mutagenesis of LpGT, several residues essential for binding of the UDP-glucose-donor and EF1A-acceptor substrates were identified, which also affected L. pneumophila virulence as demonstrated by microinjection studies. Together, these results suggested that a positively charged EF1A loop binds to a negatively charged conserved groove on the LpGT structure, and that two asparagine residues are essential for catalysis. Furthermore, we showed that two further L. pneumophila glycosyltransferases possessed the conserved UDP-glucose-binding sites and EF1A-binding grooves, and are, like LpGT, translocated into the macrophage through the Icm/Dot (intracellular multiplication/defect in organelle trafficking) system. Molecular mechanism of elongation factor 1A inhibition by a Legionella pneumophila glycosyltransferase.,Hurtado-Guerrero R, Zusman T, Pathak S, Ibrahim AF, Shepherd S, Prescott A, Segal G, van Aalten DM Biochem J. 2010 Feb 24;426(3):281-92. PMID:20030628 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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