2y24

STRUCTURAL BASIS FOR SUBSTRATE RECOGNITION BY ERWINIA CHRYSANTHEMI GH5 GLUCURONOXYLANASE

spinqualitylabelsall models 2y24, resolution 1.39Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2y24 is a 1 chain structure with sequence from "erwinia_carotovora_var._chrysanthemi"_(burkholder_et_al._1953)_dye_1969 "erwinia carotovora var. chrysanthemi" (burkholder et al. 1953) dye 1969. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GCV, IMD, PG4, PGE, XYP Related: 1nof Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Xylanase A from the phytopathogenic bacterium Erwinia chrysanthemi is classified as a glycoside hydrolase family 30 enzyme (previously in family 5) and is specialized for degradation of glucuronoxylan. The recombinant enzyme was crystallized with the aldotetraouronic acid beta-d-xylopyranosyl-(1-->4)-[4-O-methyl-alpha-d-glucuronosyl-(1-->2)]-bet a-d-xylopyranosyl-(1-->4)-d-xylose as a ligand. The crystal structure of the enzyme-ligand complex was solved at 1.39 A resolution. The ligand xylotriose moiety occupies subsites -1, -2 and -3, whereas the methyl glucuronic acid residue attached to the middle xylopyranosyl residue of xylotriose is bound to the enzyme through hydrogen bonds to five amino acids and by the ionic interaction of the methyl glucuronic acid carboxylate with the positively charged guanidinium group of Arg293. The interaction of the enzyme with the methyl glucuronic acid residue appears to be indispensable for proper distortion of the xylan chain and its effective hydrolysis. Such a distortion does not occur with linear beta-1,4-xylooligosaccharides, which are hydrolyzed by the enzyme at a negligible rate. Database Structural and experimental data are available in the Protein Data Bank database under accession number 2y24 [45]. Structural basis for substrate recognition by Erwinia chrysanthemi GH30 glucuronoxylanase.,Urbanikova L, Vrsanska M, Morkeberg Krogh KB, Hoff T, Biely P FEBS J. 2011 Apr 19. doi: 10.1111/j.1742-4658.2011.08127.x. PMID:21501386 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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