5zcn: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 5zcn is ~~ON HOLD until Paper~~ Publication

==Solution NMR structure of a new lasso peptide brevunsin==

== Structural highlights ==

<table><tr><td colspan='2'>[[5zcn]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZCN FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zcn OCA], [http://pdbe.org/5zcn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zcn RCSB], [http://www.ebi.ac.uk/pdbsum/5zcn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zcn ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

A shuttle vector pHSG396Sp was constructed to perform gene expression using Sphingomonas subterranea as a host. A new lasso peptide biosynthetic gene cluster, derived from Brevundimonas diminuta, was amplified by PCR and integrated to afford a expression vector pHSG396Sp-12697L. The new lasso peptide brevunsin was successfully produced by S. subterranea, harboring the expression vector, with a high production yield (10.2 mg from 1 L culture). The chemical structure of brevunsin was established by NMR and MS/MS experiments. Based on the information obtained from the NOE experiment, the three-dimensional structure of brevunsin was determined, which indicated that brevunsin possessed a typical lasso structure. This expression vector system provides a new heterologous production method for unexplored lasso peptides that are encoded by bacterial genomes.

~~Authors: Hemmi, H~~., Kodani, S., Miyake, Y., Kaweewan, I., Nakagawa, H.

Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea.,Kodani S, Hemmi H, Miyake Y, Kaweewan I, Nakagawa H J Ind Microbiol Biotechnol. 2018 Nov;45(11):983-992. doi:, 10.1007/s10295-018-2077-6. Epub 2018 Sep 6. PMID:30191430<ref>PMID:30191430</ref>

~~Description: Solution NMR structure~~ of ~~a new lasso peptide brevunsin~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

~~[[Category~~: ~~Unreleased Structures]]~~

</div>

~~[[Category: Kodani, S]]~~

== References ==

__TOC__

</StructureSection>

[[Category: Hemmi, H]]

[[Category: Kaweewan, I]]

[[Category: Kodani, S]]

[[Category: Miyake, Y]]

[[Category: Nakagawa, H]]

[[Category: Brevunsin]]

[[Category: Lasso peptide]]

[[Category: Sphingomonas subterranea]]

[[Category: Unknown function]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5zcn is ON HOLD  until Paper Publication
+==Solution NMR structure of a new lasso peptide brevunsin==
+<StructureSection load='5zcn' size='340' side='right' caption='[[5zcn]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5zcn]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZCN FirstGlance]. <br>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zcn OCA], [http://pdbe.org/5zcn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zcn RCSB], [http://www.ebi.ac.uk/pdbsum/5zcn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zcn ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+A shuttle vector pHSG396Sp was constructed to perform gene expression using Sphingomonas subterranea as a host. A new lasso peptide biosynthetic gene cluster, derived from Brevundimonas diminuta, was amplified by PCR and integrated to afford a expression vector pHSG396Sp-12697L. The new lasso peptide brevunsin was successfully produced by S. subterranea, harboring the expression vector, with a high production yield (10.2 mg from 1 L culture). The chemical structure of brevunsin was established by NMR and MS/MS experiments. Based on the information obtained from the NOE experiment, the three-dimensional structure of brevunsin was determined, which indicated that brevunsin possessed a typical lasso structure. This expression vector system provides a new heterologous production method for unexplored lasso peptides that are encoded by bacterial genomes.
-Authors: Hemmi, H., Kodani, S., Miyake, Y., Kaweewan, I., Nakagawa, H.
+Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea.,Kodani S, Hemmi H, Miyake Y, Kaweewan I, Nakagawa H J Ind Microbiol Biotechnol. 2018 Nov;45(11):983-992. doi:, 10.1007/s10295-018-2077-6. Epub 2018 Sep 6. PMID:30191430<ref>PMID:30191430</ref>
-Description: Solution NMR structure of a new lasso peptide brevunsin
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Kodani, S]]
+<div class="pdbe-citations 5zcn" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Hemmi, H]]
 [[Category: Kaweewan, I]]
+[[Category: Kodani, S]]
 [[Category: Miyake, Y]]
 [[Category: Nakagawa, H]]
+[[Category: Brevunsin]]
+[[Category: Lasso peptide]]
+[[Category: Sphingomonas subterranea]]
+[[Category: Unknown function]]