6h1k: Difference between revisions
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The | ==The major G-quadruplex form of HIV-1 LTR== | ||
<StructureSection load='6h1k' size='340' side='right' caption='[[6h1k]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6h1k]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H1K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H1K FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h1k OCA], [http://pdbe.org/6h1k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h1k RCSB], [http://www.ebi.ac.uk/pdbsum/6h1k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h1k ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Nucleic acids can form noncanonical four-stranded structures called G-quadruplexes. G-quadruplex-forming sequences are found in several genomes including human and viruses. Previous studies showed that the G-rich sequence located in the U3 promoter region of the HIV-1 long terminal repeat (LTR) folds into a set of dynamically interchangeable G-quadruplex structures. G-quadruplexes formed in the LTR could act as silencer elements to regulate viral transcription. Stabilization of LTR G-quadruplexes by G-quadruplex-specific ligands resulted in decreased viral production, suggesting the possibility of targeting viral G-quadruplex structures for antiviral purposes. Among all the G-quadruplexes formed in the LTR sequence, LTR-III was shown to be the major G-quadruplex conformation in vitro. Here we report the NMR structure of LTR-III in K(+) solution, revealing the formation of a unique quadruplex-duplex hybrid consisting of a three-layer (3 + 1) G-quadruplex scaffold, a 12-nt diagonal loop containing a conserved duplex-stem, a 3-nt lateral loop, a 1-nt propeller loop, and a V-shaped loop. Our structure showed several distinct features including a quadruplex-duplex junction, representing an attractive motif for drug targeting. The structure solved in this study may be used as a promising target to selectively impair the viral cycle. | |||
Major G-Quadruplex Form of HIV-1 LTR Reveals a (3 + 1) Folding Topology Containing a Stem-Loop.,Butovskaya E, Heddi B, Bakalar B, Richter SN, Phan AT J Am Chem Soc. 2018 Oct 9. doi: 10.1021/jacs.8b05332. PMID:30299955<ref>PMID:30299955</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6h1k" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bakalar, B]] | |||
[[Category: Butovskaya, E]] | |||
[[Category: Heddi, B]] | |||
[[Category: Phan, A T]] | |||
[[Category: Richter, S N]] | |||
[[Category: Dna]] | |||
[[Category: G-quadruplex structure]] | |||
[[Category: Hiv-1 ltr]] | |||
[[Category: Stem loop]] | |||
