3bb1

Crystal structure of Toc34 from Pisum sativum in complex with Mg2+ and GMPPNP

spinqualitylabelsall models 3bb1, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3bb1 is a 8 chain structure with sequence from Garden pea. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GNP, GOL, MG, PGE Related: 3bb3, 3bb4 Gene: TOC34 (Garden pea) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [TOC34_PEA] GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope. The small GTPase Toc33/34 can homodimerize, but the regulation of this interaction has remained elusive. We show that dimerization is independent of nucleotide loading state, based on crystal structures of dimeric Pisum sativum Toc34 and monomeric Arabidopsis thaliana Toc33. An arginine residue is--in the dimer--positioned to resemble a GAP arginine finger. However, GTPase activation by dimerization is sparse and active site features do not explain catalysis, suggesting that the homodimer requires an additional factor as coGAP. Access to the catalytic center and an unusual switch I movement in the dimeric structure support this finding. Potential binding sites for interactions within the Toc translocon or with precursor proteins can be derived from the structures. The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures.,Koenig P, Oreb M, Hofle A, Kaltofen S, Rippe K, Sinning I, Schleiff E, Tews I Structure. 2008 Apr;16(4):585-96. PMID:18400179 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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