Sandbox Reserved 1471: Difference between revisions

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[[Image:Peroxidase activity site.jpg|thumb|Figure 2: Peroxidase activity site from PDB file 5KIR.]]

COX-1 is made up of 576 amino acids and COX-2 is made up of 581 amino acids. (Rouzer) The sequences match with 60% identical amino acids. (Rouzer) The structures of these isozymes are nearly superimposable with three domains each. The <scene name='80/800650/Membrane_bound_domain/1'>membrane binding domain</scene> made up of residues 73-116, goes through half of the lipid bilayer. (Picot) These residues are hydrophobic because they are in a hydrophobic environment with the fatty acid tails of the lipids surrounding this domain. Residues 117-587 make up the <scene name='80/800650/Catalytic_domain/2'>catalytic domain</scene>. (Picot) The catalytic domain has two catalytic active sites for each of the catalytic activities of cyclooxygenase and peroxidase. The third domain is similar to an <scene name='80/800650/Egf_domain/1'>epidermal growth factor (EGF) domain</scene>.(Picot) The function of the EGF domain is not fully understood, but it is speculated to help with structure stability and protein-protein interactions.(Picot) This domain is linked to the catalytic domain by disulfide bridges between Cys 37- Cys 159. (Picot)

===Domains===

COX-1 is made up of 576 amino acids and COX-2 is made up of 581 amino acids.<ref name="Rouzer" /> The sequences match with 60% identical amino acids.<ref name="Rouzer" /> The structures of these isozymes are nearly superimposable with three domains each. The <scene name='80/800650/Membrane_bound_domain/1'>membrane binding domain</scene> made up of residues 73-116, goes through half of the lipid bilayer.<ref name="Picot" /> These residues are hydrophobic because they are in a hydrophobic environment with the fatty acid tails of the lipids surrounding this domain. Residues 117-587 make up the <scene name='80/800650/Catalytic_domain/2'>catalytic domain</scene>.<ref name="Picot" /> The catalytic domain has two catalytic active sites for each of the catalytic activities of cyclooxygenase and peroxidase. The third domain is similar to an <scene name='80/800650/Egf_domain/1'>epidermal growth factor (EGF) domain</scene>.<ref name="Picot" /> The function of the EGF domain is not fully understood, but it is speculated to help with structure stability and protein-protein interactions.<ref name="Picot" /> This domain is linked to the catalytic domain by disulfide bridges between Cys 37- Cys 159.<ref name="Picot" />

===Heteroatoms of the PDB file 5KIR<ref name="Orlando" />===

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@@ Line 23: / Line 23: @@
 [[Image:Peroxidase activity site.jpg|thumb|Figure 2: Peroxidase activity site from PDB file 5KIR.]]
-COX-1 is made up of 576 amino acids and COX-2 is made up of 581 amino acids. (Rouzer) The sequences match with 60% identical amino acids. (Rouzer) The structures of these isozymes are nearly superimposable with three domains each. The <scene name='80/800650/Membrane_bound_domain/1'>membrane binding domain</scene> made up of residues 73-116, goes through half of the lipid bilayer. (Picot)  These residues are hydrophobic because they are in a hydrophobic environment with the fatty acid tails of the lipids surrounding this domain. Residues 117-587 make up the <scene name='80/800650/Catalytic_domain/2'>catalytic domain</scene>. (Picot) The catalytic domain has two catalytic active sites for each of the catalytic activities of cyclooxygenase and peroxidase. The third domain is similar to an <scene name='80/800650/Egf_domain/1'>epidermal growth factor (EGF) domain</scene>.(Picot) The function of the EGF domain is not fully understood, but it is speculated to help with structure stability and protein-protein interactions.(Picot) This domain is linked to the catalytic domain by disulfide bridges between Cys 37- Cys 159. (Picot)
+===Domains===
+COX-1 is made up of 576 amino acids and COX-2 is made up of 581 amino acids.<ref name="Rouzer" /> The sequences match with 60% identical amino acids.<ref name="Rouzer" /> The structures of these isozymes are nearly superimposable with three domains each. The <scene name='80/800650/Membrane_bound_domain/1'>membrane binding domain</scene> made up of residues 73-116, goes through half of the lipid bilayer.<ref name="Picot" /> These residues are hydrophobic because they are in a hydrophobic environment with the fatty acid tails of the lipids surrounding this domain. Residues 117-587 make up the <scene name='80/800650/Catalytic_domain/2'>catalytic domain</scene>.<ref name="Picot" /> The catalytic domain has two catalytic active sites for each of the catalytic activities of cyclooxygenase and peroxidase. The third domain is similar to an <scene name='80/800650/Egf_domain/1'>epidermal growth factor (EGF) domain</scene>.<ref name="Picot" /> The function of the EGF domain is not fully understood, but it is speculated to help with structure stability and protein-protein interactions.<ref name="Picot" /> This domain is linked to the catalytic domain by disulfide bridges between Cys 37- Cys 159.<ref name="Picot" />
 ===Heteroatoms of the PDB file 5KIR<ref name="Orlando" />===