Sandbox Reserved 1475: Difference between revisions
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==Retinal Dehydrogenase Type Two Structure== | ==Retinal Dehydrogenase Type Two Structure== | ||
<Structure load='1BI9' size=' | <Structure load='1BI9' size='350' frame='true' align='right' caption='Retinal Dehydrogenase Type Two with NAD bound (PDB entry [[1bi9]])' scene=" /> | ||
This molecular structure is Retinal Dehydrogenase Type Two (RalDH2) which was extracted from ''Rattus norvegicus''.<ref name="Lamb AL, Newcomber ME">PMID:10320326</ref> This enzyme is part of the super family Aldehyde Dehydrogenase. The sample was a crystallization of a clone of RalDH2 that was given as a gift from J. L. Napoli. It has been shown that RalDH2, the cellular binding protein type I, and the retinol binding protein receptor all co-localize in tissues that require Vitamin A (retinol) for normal development in rat embryos post-gastrulation, as discussed by "personal communication" to the authors of the ''"The Structure of Retinal Dehydrogenase Type II at 2.7 Angstrom Resolution: Implications for Retinal Specificity"''.<ref name="Lamb AL, Newcomber ME" /> RalDH2 is the enzyme that produces retinoic acid in mouse embryos during gastrulation, and is found to be surrounding the primitive node, which is where retinal is converted into retinoic acid.<ref name ="Ruberte E">PMID:1849812</ref> | This molecular structure is Retinal Dehydrogenase Type Two (RalDH2) which was extracted from ''Rattus norvegicus''.<ref name="Lamb AL, Newcomber ME">PMID:10320326</ref> This enzyme is part of the super family Aldehyde Dehydrogenase. The sample was a crystallization of a clone of RalDH2 that was given as a gift from J. L. Napoli. It has been shown that RalDH2, the cellular binding protein type I, and the retinol binding protein receptor all co-localize in tissues that require Vitamin A (retinol) for normal development in rat embryos post-gastrulation, as discussed by "personal communication" to the authors of the ''"The Structure of Retinal Dehydrogenase Type II at 2.7 Angstrom Resolution: Implications for Retinal Specificity"''.<ref name="Lamb AL, Newcomber ME" /> RalDH2 is the enzyme that produces retinoic acid in mouse embryos during gastrulation, and is found to be surrounding the primitive node, which is where retinal is converted into retinoic acid.<ref name ="Ruberte E">PMID:1849812</ref> | ||
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== Structural highlights == | == Structural highlights == | ||
<Structure load='1bi9' size=' | <Structure load='1bi9' size='350' frame='true' align='left' caption='[[Figure 2]] Nucleotide-Binding domain - orange, Catalytic domain - green, Tetramerization domain - blue (PDB entry [[1bi9]])' scene='80/800654/3_domains/3' /> | ||
[[Image:3 Domains of RalDH2.png|thumb|upright=1.5| [[Figure 1]] Nucleotide-binding domain - orange, Catalytic domain - green, Tetramerization domain - blue. Imagine modified in Chimera from (PDB entry [[1bi9]]). Chain D shown with NAD substrate shown in pink]] | [[Image:3 Domains of RalDH2.png|thumb|upright=1.5| [[Figure 1]] Nucleotide-binding domain - orange, Catalytic domain - green, Tetramerization domain - blue. Imagine modified in Chimera from (PDB entry [[1bi9]]). Chain D shown with NAD substrate shown in pink]] | ||
