3biw: Difference between revisions
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|PDB= 3biw |SIZE=350|CAPTION= <scene name='initialview01'>3biw</scene>, resolution 3.500Å | |PDB= 3biw |SIZE=350|CAPTION= <scene name='initialview01'>3biw</scene>, resolution 3.500Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= Nlgn1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]), Nrxn1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | |GENE= Nlgn1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]), Nrxn1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[3bix|3BIX]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3biw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3biw OCA], [http://www.ebi.ac.uk/pdbsum/3biw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3biw RCSB]</span> | |||
}} | }} | ||
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[[Category: Strop, P.]] | [[Category: Strop, P.]] | ||
[[Category: Sudhof, T C.]] | [[Category: Sudhof, T C.]] | ||
[[Category: alpha-beta hydrolase]] | [[Category: alpha-beta hydrolase]] | ||
[[Category: alternative promoter usage]] | [[Category: alternative promoter usage]] | ||
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[[Category: transmembrane]] | [[Category: transmembrane]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:26:45 2008'' | ||
Revision as of 02:26, 31 March 2008
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| 3biw, resolution 3.500Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | CA, NAG | ||||||||||||
| Gene: | Nlgn1 (Rattus norvegicus), Nrxn1 (Rattus norvegicus) | ||||||||||||
| Related: | 3BIX
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of the Neuroligin-1/Neurexin-1beta synaptic adhesion complex
Overview
Neurexins and neuroligins provide trans-synaptic connectivity by the Ca2+-dependent interaction of their alternatively spliced extracellular domains. Neuroligins specify synapses in an activity-dependent manner, presumably by binding to neurexins. Here, we present the crystal structures of neuroligin-1 in isolation and in complex with neurexin-1 beta. Neuroligin-1 forms a constitutive dimer, and two neurexin-1 beta monomers bind to two identical surfaces on the opposite faces of the neuroligin-1 dimer to form a heterotetramer. The neuroligin-1/neurexin-1 beta complex exhibits a nanomolar affinity and includes a large binding interface that contains bound Ca2+. Alternatively spliced sites in neurexin-1 beta and in neuroligin-1 are positioned nearby the binding interface, explaining how they regulate the interaction. Structure-based mutations of neuroligin-1 at the interface disrupt binding to neurexin-1 beta, but not the folding of neuroligin-1 and confirm the validity of the binding interface of the neuroligin-1/neurexin-1 beta complex. Our results provide molecular insights for understanding the role of cell-adhesion proteins in synapse function.
About this Structure
3BIW is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Structures of neuroligin-1 and the neuroligin-1/neurexin-1 beta complex reveal specific protein-protein and protein-Ca2+ interactions., Arac D, Boucard AA, Ozkan E, Strop P, Newell E, Sudhof TC, Brunger AT, Neuron. 2007 Dec 20;56(6):992-1003. PMID:18093522
Page seeded by OCA on Mon Mar 31 05:26:45 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Protein complex
- Rattus norvegicus
- Arac, D.
- Boucard, A A.
- Brunger, A T.
- Newell, E.
- Ozkan, E.
- Strop, P.
- Sudhof, T C.
- Alpha-beta hydrolase
- Alternative promoter usage
- Alternative splicing
- Cell adhesion
- Cell adhesion/cell adhesion complex
- Cell junction
- Esterase domain
- Glycoprotein
- Lns domain
- Membrane
- Postsynaptic cell membrane
- Protein-protein complex
- Synapse
- Transmembrane
