Sandbox Reserved 1477: Difference between revisions

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==Introduction==

The topic is ~~going to be~~ the ~~structural~~ study of the enzyme '''glucose oxidase''' from aspergillus niger. It is not an enzyme from human body. Instead, it is found in the cells of fungus. These types of enzymes catalysts the oxidation of beta-D-glucose to δ-gluconolactone and H2O2 (which is not a reaction happening in human body) <ref>PMID:10216293</ref>. I don't think there is any medical application for this certain enzyme since it is not even an enzyme from human body. The only possible medical application might be producing medicines with this type of reaction.

The topic is the study of the enzyme '''glucose oxidase''' (GOX) from '''aspergillus niger'''. It is not an enzyme from human body. Instead, it is found in the cells of fungus. These types of enzymes catalysts the oxidation of beta-D-glucose to δ-gluconolactone and H2O2 (which is not a reaction happening in human body) <ref>PMID:10216293</ref>.

Two PDB ~~codes~~ mentioned by the essay, 1cf3 and 1gpe, which are two glucose oxidases from different organisms. They have exactly the same types of ligands, which ~~might mean there is some functional issue with~~ the ~~present of those ligands, especially the dimer~~ of beta-D-glucose ~~(MAN)~~. This essay was published back to 1999, which is fairly old in the scale of ~~proteomic~~.

GOX has a “considerable commercial importance” in biosensors according to the essay. This type of enzyme is wildly used in biosensor. Since it could react with glucose with a great selectivity and produce hydrogen peroxide, the amount of hydrogen peroxide is a function of the initial amount of the glucose ideally. For is reason, it is used for quantitively measuring the amount of concentration of glucose, or in a simpler way, measuring the concentration of blood glucose <ref>PMID: 24907743</ref>. Thus, it could be (actually had already been) widely used in quick blood glucose tests, which is essential to those who have diabetes. However, the usage of this enzyme is not exactly limited to the medical area. A good method of reflecting the concentration of glucose could also be used in food industry in measuring and controlling the sugar amount in food samples since most types of natural-sourced carbohydrate in food industry, such as sucrose and lactose, contain glucose <ref>PMID: 19374943</ref>.

Two PDB files mentioned in the essay, 1cf3 and 1gpe, which are two glucose oxidases from different organisms, ''aspergillus niger''' and '''Penicillium Amagasakiense'''. They have exactly the same types of ligands. The most important ligand is FAD cofactor, which assists the oxidation of beta-D-glucose. This essay was published back to 1999, which is fairly old in the scale of science.

== Function ==

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 ==Introduction==
-The topic is going to be the structural study of the enzyme '''glucose oxidase''' from aspergillus niger. It is not an enzyme from human body. Instead, it is found in the cells of fungus. These types of enzymes catalysts the oxidation of beta-D-glucose to δ-gluconolactone and H2O2 (which is not a reaction happening in human body) <ref>PMID:10216293</ref>. I don't think there is any medical application for this certain enzyme since it is not even an enzyme from human body. The only possible medical application might be producing medicines with this type of reaction.
+The topic is the study of the enzyme '''glucose oxidase''' (GOX) from '''aspergillus niger'''. It is not an enzyme from human body. Instead, it is found in the cells of fungus. These types of enzymes catalysts the oxidation of beta-D-glucose to δ-gluconolactone and H2O2 (which is not a reaction happening in human body) <ref>PMID:10216293</ref>.
-Two PDB codes mentioned by the essay, 1cf3 and 1gpe, which are two glucose oxidases from different organisms. They have exactly the same types of ligands, which might mean there is some functional issue with the present of those ligands, especially the dimer of beta-D-glucose (MAN). This essay was published back to 1999, which is fairly old in the scale of proteomic.
+GOX has a “considerable commercial importance” in biosensors according to the essay. This type of enzyme is wildly used in biosensor. Since it could react with glucose with a great selectivity and produce hydrogen peroxide, the amount of hydrogen peroxide is a function of the initial amount of the glucose ideally. For is reason, it is used for quantitively measuring the amount of concentration of glucose, or in a simpler way, measuring the concentration of blood glucose <ref>PMID: 24907743</ref>. Thus, it could be (actually had already been) widely used in quick blood glucose tests, which is essential to those who have diabetes. However, the usage of this enzyme is not exactly limited to the medical area. A good method of reflecting the concentration of glucose could also be used in food industry in measuring and controlling the sugar amount in food samples since most types of natural-sourced carbohydrate in food industry, such as sucrose and lactose, contain glucose <ref>PMID: 19374943</ref>.
+Two PDB files mentioned in the essay, 1cf3 and 1gpe, which are two glucose oxidases from different organisms, ''aspergillus niger''' and '''Penicillium Amagasakiense'''. They have exactly the same types of ligands. The most important ligand is FAD cofactor, which assists the oxidation of beta-D-glucose. This essay was published back to 1999, which is fairly old in the scale of science.
 == Function ==