Sandbox Reserved 1501: Difference between revisions
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The amino acid sequence in the heme binding region was first determined by Guidard | The amino acid sequence in the heme binding region was first determined by Guidard ''et al''. (1974). | ||
For every subunit of the wild type protein form, the crystallized preparation analysis determined a molecular weight of the chain of 36 kD (Appleby and Morton<ref>PMID: 13638255</ref>, 1959) and the chain of 21 kD (Jacq and Lederer<ref>PMID: 4152980</ref>, 1974). | For every subunit of the wild type protein form, the crystallized preparation analysis determined a molecular weight of the chain of 36 kD (Appleby and Morton<ref>PMID: 13638255</ref>, 1959) and the chain of 21 kD (Jacq and Lederer<ref>PMID: 4152980</ref>, 1974). | ||
The sulfite adduct recombinant enzyme produced when expressed in ‘’E. coli’’ was also crystallized (Tegoni and Cambillau<ref>PMID: 8003966</ref>, 1994) so key active site residues could be identified and comparisons with the mutant protein. | The sulfite adduct recombinant enzyme produced when expressed in ‘’E. coli’’ was also crystallized (Tegoni and Cambillau<ref>PMID: 8003966</ref>, 1994) so key active site residues could be identified and comparisons with the mutant protein. | ||
