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'''Unreleased structure'''


The entry 6fix is ON HOLD
==antitoxin GraA in complex with its operator==
<StructureSection load='6fix' size='340' side='right' caption='[[6fix]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6fix]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FIX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FIX FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fix OCA], [http://pdbe.org/6fix PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fix RCSB], [http://www.ebi.ac.uk/pdbsum/6fix PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fix ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The graTA operon from Pseudomonas putida encodes a toxin-antitoxin module with an unusually moderate toxin. Here, the production, SAXS analysis and crystallization of the antitoxin GraA, the GraTA complex and the complex of GraA with a 33 bp operator fragment are reported. GraA forms a homodimer in solution and crystallizes in space group P21, with unit-cell parameters a = 66.9, b = 48.9, c = 62.7 A, beta = 92.6 degrees . The crystals are likely to contain two GraA dimers in the asymmetric unit and diffract to 1.9 A resolution. The GraTA complex forms a heterotetramer in solution. Crystals of the GraTA complex diffracted to 2.2 A resolution and are most likely to contain a single heterotetrameric GraTA complex in the asymmetric unit. They belong to space group P41 or P43, with unit-cell parameters a = b = 56.0, c = 128.2 A. The GraA-operator complex consists of a 33 bp operator region that binds two GraA dimers. It crystallizes in space group P31 or P32, with unit-cell parameters a = b = 105.6, c = 149.9 A. These crystals diffract to 3.8 A resolution.


Authors: Talavera, A., Loris, R.
Production, biophysical characterization and crystallization of Pseudomonas putida GraA and its complexes with GraT and the graTA operator.,Talavera A, Tamman H, Ainelo A, Hadaei S, Garcia-Pino A, Horak R, Konijnenberg A, Loris R Acta Crystallogr F Struct Biol Commun. 2017 Aug 1;73(Pt 8):455-462. doi:, 10.1107/S2053230X17009438. Epub 2017 Jul 26. PMID:28777088<ref>PMID:28777088</ref>


Description: antitoxin GraA in complex with its operator
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 6fix" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Loris, R]]
[[Category: Loris, R]]
[[Category: Talavera, A]]
[[Category: Talavera, A]]
[[Category: Antitoxin]]
[[Category: Dna]]
[[Category: Graa]]
[[Category: Grat]]
[[Category: Higa]]
[[Category: Higb]]
[[Category: Operator]]
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