| Structural highlights
5ekb is a 1 chain structure with sequence from Geoka. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | FE, MN3, PLM |
| Related: | 4hr0, 4hr4, 4hr5, 4xbv, 4xbw, 4xb9, 5dco, 5dcr, 5dcs |
| Gene: | GK2771 (GEOKA) |
| Activity: | Ribonucleoside-diphosphate reductase, with EC number 1.17.4.1 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
A manganese/iron cofactor which performs multi-electron oxidative chemistry is found in two classes of ferritin-like proteins, the small subunit (R2) of class Ic ribonucleotide reductase (R2c) and the R2-like ligand-binding oxidase (R2lox). It is unclear how a heterodimeric Mn/Fe metallocofactor is assembled in these two related proteins as opposed to a homodimeric Fe/Fe cofactor, especially considering the structural similarity and proximity of the two metal-binding sites in both protein scaffolds and the similar first coordination sphere ligand preferences of MnII and FeII. Using EPR and Mossbauer spectroscopies as well as X-ray anomalous dispersion, we examined metal loading and cofactor activation of both proteins in vitro (in solution). We find divergent cofactor assembly mechanisms for the two systems. In both cases, excess MnII promotes heterobimetallic cofactor assembly. In the absence of FeII, R2c cooperatively binds MnII at both metal sites, whereas R2lox does not readily bind MnII at either site. Heterometallic cofactor assembly is favored at substoichiometric FeII concentrations in R2lox. FeII and MnII likely bind to the protein in a stepwise fashion, with FeII binding to site 2 initiating cofactor assembly. In R2c, however, heterometallic assembly is presumably achieved by the displacement of MnII by FeII at site 2. The divergent metal loading mechanisms are correlated with the putative in vivo functions of R2c and R2lox, and most likely with the intracellular MnII/FeII concentrations in the host organisms from which they were isolated.
Divergent assembly mechanisms of the manganese/iron cofactors in R2lox and R2c proteins.,Kutin Y, Srinivas V, Fritz M, Kositzki R, Shafaat HS, Birrell J, Bill E, Haumann M, Lubitz W, Hogbom M, Griese JJ, Cox N J Inorg Biochem. 2016 Apr 16. pii: S0162-0134(16)30104-0. doi:, 10.1016/j.jinorgbio.2016.04.019. PMID:27138102[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kutin Y, Srinivas V, Fritz M, Kositzki R, Shafaat HS, Birrell J, Bill E, Haumann M, Lubitz W, Hogbom M, Griese JJ, Cox N. Divergent assembly mechanisms of the manganese/iron cofactors in R2lox and R2c proteins. J Inorg Biochem. 2016 Apr 16. pii: S0162-0134(16)30104-0. doi:, 10.1016/j.jinorgbio.2016.04.019. PMID:27138102 doi:https://dx.doi.org/10.1016/j.jinorgbio.2016.04.019
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