3w0d

Structure of elastase inhibitor AFUEI (cyrstal form I)

spinqualitylabelsall models 3w0d, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3w0d is a 2 chain structure with sequence from Aspergillus fumigatus af293. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 3w0e Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [IELA_ASPFU] Elastase inhibitor. Non-competitive inhibitor of A.fumigatus elastase with a Ki of 1.6 nM. Inhibits the fibrinogenase and collagenase activities of A.fumigatus elastase. Inhibitor of A.flavus elastase and human leukocyte elastase, and weakly inhibits porcine pancreatic elastase. Does not inhibit elastase from P.aeruginosa or C.atrox venom. Publication Abstract from PubMed Elastase from Aspergillus sp. is an important factor for aspergillosis. AFUEI is an inhibitor of the elastase derived from Aspergillus fumigatus. AFUEI is a member of the I78 inhibitor family, and has a high inhibitory activity against elastases of Aspergillus fumigatus and Aspergillus flavus, human neutrophil elastase and bovine chymotrypsin, but does not inhibit bovine trypsin. Here we report the crystal structure of AFUEI in two crystal forms. AFUEI is a wedge-shaped protein composed of an extended loop and a scaffold protein core. The structure of AFUEI shows remarkable similarity to serine protease inhibitors of the potato inhibitor I family, although they are classified into different inhibitor families. A structural comparison with the potato I family inhibitors suggests that the extended loop of AFUEI corresponds to the binding loop of the potato inhibitor I family and AFUEI inhibits its cognate proteases through the same mechanism as the Potato I family inhibitors. X-ray structure analysis and characterization of AFUEI, an elastase inhibitor from Aspergillus fumigatus.,Sakuma M, Imada K, Okumura Y, Uchiya KI, Yamashita N, Ogawa K, Hijikata A, Shirai T, Homma M, Nikai T J Biol Chem. 2013 May 2. PMID:23640894 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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