4awn

Structure of recombinant human DNase I (rhDNaseI) in complex with Magnesium and Phosphate.

spinqualitylabelsall models 4awn, resolution 1.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4awn is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BTB, CA, MG, NAG, PO4 Activity: Deoxyribonuclease I, with EC number 3.1.21.1 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Disease [DNAS1_HUMAN] Systemic lupus erythematosus. Disease susceptibility is associated with variations affecting the gene represented in this entry. Function [DNAS1_HUMAN] Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity). Publication Abstract from PubMed Recombinant human DNase I (Pulmozyme, dornase alfa) is used for the treatment of cystic fibrosis where it improves lung function and reduces the number of exacerbations. The physiological mechanism of action is thought to involve the reduction of the viscoelasticity of cystic fibrosis sputum by hydrolyzing high concentrations of DNA into low-molecular mass fragments. Here we describe the 1.95 A resolution crystal structure of recombinant human DNase I (rhDNase I) in complex with magnesium and phosphate ions, both bound in the active site. Complementary mutagenesis data of rhDNase I coupled to a comprehensive structural analysis of the DNase I-like superfamily argue for the key catalytic role of Asn7, which is invariant among mammalian DNase I enzymes and members of this superfamily, through stabilization of the magnesium ion coordination sphere. Overall, our combined structural and mutagenesis data suggest the occurrence of a magnesium-assisted pentavalent phosphate transition state in human DNase I during catalysis, where Asp168 may play a key role as a general catalytic base. The Structure of Human DNase I Bound to Magnesium and Phosphate Ions Points to a Catalytic Mechanism Common to Members of the DNase I-like Superfamily.,Parsiegla G, Noguere C, Santell L, Lazarus RA, Bourne Y Biochemistry. 2012 Dec 21;51(51):10250-8. doi: 10.1021/bi300873f. Epub 2012 Dec, 13. PMID:23215638 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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