6n9y: Difference between revisions
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==Atomic structure of Non-Structural protein 1 of bluetongue virus== | |||
<StructureSection load='6n9y' size='340' side='right'caption='[[6n9y]], [[Resolution|resolution]] 4.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6n9y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bluetongue_virus_23 Bluetongue virus 23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N9Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N9Y FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n9y OCA], [http://pdbe.org/6n9y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n9y RCSB], [http://www.ebi.ac.uk/pdbsum/6n9y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n9y ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bluetongue virus (BTV) non-structural protein 1 (NS1) regulates viral protein synthesis and exists as tubular and non-tubular forms in infected cells, but how tubules assemble and how protein synthesis is regulated are unknown. Here, we report near-atomic resolution structures of two NS1 tubular forms determined by cryo-electron microscopy. The two tubular forms are different helical assemblies of the same NS1 monomer, consisting of an amino-terminal foot, a head and body domains connected to an extended carboxy-terminal arm, which wraps atop the head domain of another NS1 subunit through hydrophobic interactions. Deletion of the C terminus prevents tubule formation but not viral replication, suggesting an active non-tubular form. Two zinc-finger-like motifs are present in each NS1 monomer, and tubules are disrupted by divalent cation chelation and restored by cation addition, including Zn(2+), suggesting a regulatory role of divalent cations in tubule formation. In vitro luciferase assays show that the NS1 non-tubular form upregulates BTV mRNA translation, whereas zinc-finger disruption decreases viral mRNA translation, tubule formation and virus replication, confirming a functional role for the zinc-fingers. Thus, the non-tubular form of NS1 is sufficient for viral protein synthesis and infectious virus replication, and the regulatory mechanism involved operates through divalent cation-dependent conversion between the non-tubular and tubular forms. | |||
Atomic structure of the translation regulatory protein NS1 of bluetongue virus.,Kerviel A, Ge P, Lai M, Jih J, Boyce M, Zhang X, Zhou ZH, Roy P Nat Microbiol. 2019 Feb 18. pii: 10.1038/s41564-019-0369-x. doi:, 10.1038/s41564-019-0369-x. PMID:30778144<ref>PMID:30778144</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6n9y" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bluetongue virus 23]] | |||
[[Category: Large Structures]] | |||
[[Category: Boyce, M]] | |||
[[Category: Ge, P]] | |||
[[Category: Jih, J]] | |||
[[Category: Kerviel, A]] | |||
[[Category: Lai, M]] | |||
[[Category: Roy, P]] | |||
[[Category: Zhang, X]] | |||
[[Category: Zhou, Z H]] | |||
[[Category: Bluetongue virus non-structural protein 1]] | |||
[[Category: Viral protein]] | |||
Revision as of 12:16, 13 March 2019
Atomic structure of Non-Structural protein 1 of bluetongue virus
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