6a7f: Difference between revisions

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==The cryo-EM structure of filamentous bacteriophage IKe major coat protein p8 shell assembly.==

== Structural highlights ==

<table><tr><td colspan='2'>[[6a7f]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Filamentous_phage Filamentous phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A7F FirstGlance]. <br>

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== Function ==

[[http://www.uniprot.org/uniprot/CAPSD_BPIKE CAPSD_BPIKE]] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).

== Publication Abstract from PubMed ==

The filamentous bacteriophage IKe infects Escherichia coli cells bearing IncN pili. We report the cryo-electron microscopy structure of the micrometer-long IKe viral particle at a resolution of 3.4 A. The major coat protein [protein 8 (p8)] consists of 47 residues that fold into a approximately 68-A-long helix. An atomic model of the coat protein was built. Five p8 helices in a horizontal layer form a pentamer, and symmetrically neighboring p8 layers form a right-handed helical cylinder having a rise per pentamer of 16.77 A and a twist of 38.52 degrees . The inner surface of the capsid cylinder is positively charged and has direct interactions with the encapsulated circular single-stranded DNA genome, which has an electron density consistent with an unusual left-handed helix structure. Similar to capsid structures of other filamentous viruses, strong capsid packing in the IKe particle is maintained by hydrophobic residues. Despite having a different length and large sequence differences from other filamentous phages, pi-pi interactions were found between Tyr9 of one p8 and Trp29 of a neighboring p8 in IKe that are similar to interactions observed in phage M13, suggesting that, despite sequence divergence, overall structural features are maintained.

Cryo-electron microscopy structure of the filamentous bacteriophage IKe.,Xu J, Dayan N, Goldbourt A, Xiang Y Proc Natl Acad Sci U S A. 2019 Feb 28. pii: 1811929116. doi:, 10.1073/pnas.1811929116. PMID:30819888<ref>PMID:30819888</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Filamentous phage]]

[[Category: Large Structures]]

[[Category: Dayan, N]]

[[Category: Goldbourt, A]]

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@@ Line 1: / Line 1: @@
 ==The cryo-EM structure of filamentous bacteriophage IKe major coat protein p8 shell assembly.==
-<StructureSection load='6a7f' size='340' side='right' caption='[[6a7f]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
+<StructureSection load='6a7f' size='340' side='right'caption='[[6a7f]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[6a7f]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Filamentous_phage Filamentous phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A7F FirstGlance]. <br>
@@ Line 8: / Line 8: @@
 == Function ==
 [[http://www.uniprot.org/uniprot/CAPSD_BPIKE CAPSD_BPIKE]] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The filamentous bacteriophage IKe infects Escherichia coli cells bearing IncN pili. We report the cryo-electron microscopy structure of the micrometer-long IKe viral particle at a resolution of 3.4 A. The major coat protein [protein 8 (p8)] consists of 47 residues that fold into a approximately 68-A-long helix. An atomic model of the coat protein was built. Five p8 helices in a horizontal layer form a pentamer, and symmetrically neighboring p8 layers form a right-handed helical cylinder having a rise per pentamer of 16.77 A and a twist of 38.52 degrees . The inner surface of the capsid cylinder is positively charged and has direct interactions with the encapsulated circular single-stranded DNA genome, which has an electron density consistent with an unusual left-handed helix structure. Similar to capsid structures of other filamentous viruses, strong capsid packing in the IKe particle is maintained by hydrophobic residues. Despite having a different length and large sequence differences from other filamentous phages, pi-pi interactions were found between Tyr9 of one p8 and Trp29 of a neighboring p8 in IKe that are similar to interactions observed in phage M13, suggesting that, despite sequence divergence, overall structural features are maintained.
+Cryo-electron microscopy structure of the filamentous bacteriophage IKe.,Xu J, Dayan N, Goldbourt A, Xiang Y Proc Natl Acad Sci U S A. 2019 Feb 28. pii: 1811929116. doi:, 10.1073/pnas.1811929116. PMID:30819888<ref>PMID:30819888</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 6a7f" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
 __TOC__
 </StructureSection>
 [[Category: Filamentous phage]]
+[[Category: Large Structures]]
 [[Category: Dayan, N]]
 [[Category: Goldbourt, A]]