4b22

Unprecedented sculpting of DNA at abasic sites by DNA glycosylase homolog Mag2

spinqualitylabelsall models 4b22, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4b22 is a 3 chain structure with sequence from Cbs 356. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: 3DR Related: 4b21, 4b23, 4b24 Activity: DNA-3-methyladenine glycosylase II, with EC number 3.2.2.21 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [MAG2_SCHPO] Invlved in base excision repair of methyl methanesulfonate-damaged DNA by hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions. Publication Abstract from PubMed Modifications and loss of bases are frequent types of DNA lesions, often handled by the base excision repair (BER) pathway. BER is initiated by DNA glycosylases, generating abasic (AP) sites that are subsequently cleaved by AP endonucleases, which further pass on nicked DNA to downstream DNA polymerases and ligases. The coordinated handover of cytotoxic intermediates between different BER enzymes is most likely facilitated by the DNA conformation. Here, we present the atomic structure of Schizosaccharomyces pombe Mag2 in complex with DNA to reveal an unexpected structural basis for nonenzymatic AP site recognition with an unflipped AP site. Two surface-exposed loops intercalate and widen the DNA minor groove to generate a DNA conformation previously only found in the mismatch repair MutS-DNA complex. Consequently, the molecular role of Mag2 appears to be AP site recognition and protection, while possibly facilitating damage signaling by structurally sculpting the DNA substrate. Sculpting of DNA at Abasic Sites by DNA Glycosylase Homolog Mag2.,Dalhus B, Nilsen L, Korvald H, Huffman J, Forstrom RJ, McMurray CT, Alseth I, Tainer JA, Bjoras M Structure. 2012 Dec 11. pii: S0969-2126(12)00419-4. doi:, 10.1016/j.str.2012.11.004. PMID:23245849 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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