4bz4

CorA is a surface-associated copper-binding protein important in Methylomicrobium album BG8 copper acquisition

spinqualitylabelsall models 4bz4, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4bz4 is a 6 chain structure with sequence from Methylomicrobium album bg8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA, CU1, P6G, PG4 NonStd Res: KYN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed CorA is a copper repressible protein previously identified in the methanotrophic bacterium Methylomicrobium album BG8. In this work, we demonstrate that CorA is located on the cell surface and binds one copper ion per protein molecule, which, based on X-ray Absorption Near Edge Structure analysis, is in the reduced state (Cu(I)). The structure of endogenously expressed CorA was solved using X-ray crystallography. The 1.6 A three-dimensional structure confirmed the binding of copper and revealed that the copper atom was coordinated in a mononuclear binding site defined by two histidines, one water molecule, and the tryptophan metabolite, kynurenine. This arrangement of the copper-binding site is similar to that of its homologous protein MopE* from Metylococcus capsulatus Bath, confirming the importance of kynurenine for copper binding in these proteins. Our findings show that CorA has an overall fold similar to MopE, including the unique copper(I)-binding site and most of the secondary structure elements. We suggest that CorA plays a role in the M. album BG8 copper acquisition. CorA Is a Copper Repressible Surface-Associated Copper(I)-Binding Protein Produced in Methylomicrobium album BG8.,Johnson KA, Ve T, Larsen O, Pedersen RB, Lillehaug JR, Jensen HB, Helland R, Karlsen OA PLoS One. 2014 Feb 3;9(2):e87750. doi: 10.1371/journal.pone.0087750. eCollection , 2014. PMID:24498370 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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